Human BDKRB2-tGFP Stable Cell Line-CHO-K1

Name: Human BDKRB2-tGFP Stable Cell Line-CHO-K1
SKU: CSC-RG0026
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0026

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0026
Background	BDKR2 gene encodes a protein that is one of two Bradykinin receptors. The bradykinin receptor family is a group of G-protein coupled receptors whose principal ligand is the protein bradykinin. This gene encodes a receptor for bradykinin 2. The 9 aa bradykinin peptide elicits many responses including vasodilation, edema, smooth muscle spasm and pain fiber stimulation. This receptor associates with G proteins, stimulates a phosphatidy linositol calcium second messenger system.
Target Gene	BDKRB2
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuBDKRB2-tGFP
Alias	BDKRB2, BK 2, B2R, BK2, BK-2, BKR2, BRB2
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent
Morphology	Epithelial-like

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	BDKRB2 bradykinin receptor B2 [ Homo sapiens ]
Gene Symbol	BDKRB2
Synonyms	B2R; BK2; BK-2; BKR2; BRB2
Gene Description	bradykinin receptor B2
GeneID	624
Uni ProtID	P30411
mRNA Refseq	NM_000623.3
Protein Refseq	NP_000614.1
Chromosome Location	14q32.1-q32.2
Function	bradykinin receptor activity; phosphatidylinositol phospholipase C activity; protease binding; protein binding; protein heterodimerization activity; type 1 angiotensin receptor binding;
Pathway	ACE Inhibitor Pathway, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem;
MIM	113503

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Customer Reviews

Customer Q&As

What are the advantages of the Human BDKRB2-tGFP Stable Cell Line-CHO in drug screening applications?

A: The Human BDKRB2-tGFP Stable Cell Line-CHO expresses the human B-type D2 dopamine receptor (BDKRB2) stably and with a green fluorescent protein (tGFP) tag. The advantages of this cell line in drug screening include: (1) Human origin, which can more accurately simulate pharmacological responses in the human body; (2) Stable tGFP tag facilitates high-throughput screening with fluorescence imaging technology; (3) It can be used to study the signaling and drug action mechanisms of the D2 receptor; (4) It is suitable for developing new drugs targeting the D2 receptor, especially in the field of psychopharmacology.

How does the Human BDKRB2-tGFP Stable Cell Line-CHO contribute to understanding the regulation of the dopamine system in neuroscience research?

A: By stably expressing Human BDKRB2 with a tGFP tag in CHO cells, researchers can directly observe and quantify the effects of dopamine on the activation and inhibition of the D2 receptor. This cell line provides a model system to study the molecular mechanisms of dopamine signaling and the role of the dopamine system in neural development, learning, memory, and neurodegenerative diseases such as Parkinson's disease.

What is the role of the Human BDKRB2-tGFP Stable Cell Line-CHO cell line in drug toxicity testing?

A: The cell line can be used to assess the toxic effects of potential drugs on the D2 receptor. By monitoring tGFP expression levels and cell viability, researchers can quickly screen out compounds with potential toxicity to the D2 receptor, reducing risks in subsequent research.

How is the Human BDKRB2-tGFP Stable Cell Line-CHO cell line used for drug pharmacokinetics research?

A: The cell line can be used to study the binding kinetics of drugs with the D2 receptor, including the association and dissociation rates, as well as the distribution and clearance of drugs within the cell. These data are crucial for understanding the mechanism of action of drugs and optimizing drug design.

What is the high-throughput screening (HTS) strategy for the Human BDKRB2-tGFP Stable Cell Line-CHO cell line in drug discovery?

A: In HTS, the cell line can be used to rapidly screen large compound libraries to identify compounds that activate or inhibit D2 receptor activity. Fluorescence imaging technology allows for the quantitative measurement of changes in tGFP expression, assessing the impact of compounds on D2 receptor function.

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