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Cat.No. : CSC-RG0026 Host Cell: CHO-K1
| Cat. No. | CSC-RG0026 |
| Background | BDKR2 gene encodes a protein that is one of two Bradykinin receptors. The bradykinin receptor family is a group of G-protein coupled receptors whose principal ligand is the protein bradykinin. This gene encodes a receptor for bradykinin 2. The 9 aa bradykinin peptide elicits many responses including vasodilation, edema, smooth muscle spasm and pain fiber stimulation. This receptor associates with G proteins, stimulates a phosphatidy linositol calcium second messenger system. |
| Gene | BDKRB2 |
| Gene Species | Homo sapiens (Human) |
| Alias | BDKRB2, BK 2, B2R, BK2, BK-2, BKR2, BRB2 |
| Host Cell | CHO-K1 |
| Species | Cricetulus griseus (Chinese hamster) |
| Morphology | Epithelial-like |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human BDKRB2-tGFP Stable Cell Line-CHO expresses the human B-type D2 dopamine receptor (BDKRB2) stably and with a green fluorescent protein (tGFP) tag. The advantages of this cell line in drug screening include: (1) Human origin, which can more accurately simulate pharmacological responses in the human body; (2) Stable tGFP tag facilitates high-throughput screening with fluorescence imaging technology; (3) It can be used to study the signaling and drug action mechanisms of the D2 receptor; (4) It is suitable for developing new drugs targeting the D2 receptor, especially in the field of psychopharmacology.
A: By stably expressing Human BDKRB2 with a tGFP tag in CHO cells, researchers can directly observe and quantify the effects of dopamine on the activation and inhibition of the D2 receptor. This cell line provides a model system to study the molecular mechanisms of dopamine signaling and the role of the dopamine system in neural development, learning, memory, and neurodegenerative diseases such as Parkinson's disease.
A: The cell line can be used to assess the toxic effects of potential drugs on the D2 receptor. By monitoring tGFP expression levels and cell viability, researchers can quickly screen out compounds with potential toxicity to the D2 receptor, reducing risks in subsequent research.
A: The cell line can be used to study the binding kinetics of drugs with the D2 receptor, including the association and dissociation rates, as well as the distribution and clearance of drugs within the cell. These data are crucial for understanding the mechanism of action of drugs and optimizing drug design.
A: In HTS, the cell line can be used to rapidly screen large compound libraries to identify compounds that activate or inhibit D2 receptor activity. Fluorescence imaging technology allows for the quantitative measurement of changes in tGFP expression, assessing the impact of compounds on D2 receptor function.
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