Cat.No. : CSC-RG0007 Host Cell: HEK293
| Cat. No. | CSC-RG0007 |
| Background | Bradykinin receptor B2 is a G-protein coupled receptor for Bradykinin, encoded by the BDKRB2 gene in humans. The B2 receptor is a G protein-coupled receptor that stimulates phospholipase C to increase intracellular free calcium. The 9 amino acid Bradykinin peptide elicits many responses including vasodilation, edema, smooth muscle spasm and pain fiber stimulation. |
| Gene | BDKRB2 |
| Gene Species | Homo sapiens (Human) |
| Alias | BDKRB2, B2R, BK-2, BK2, BKR2, BRB2 |
| Host Cell | HEK293 |
| Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human BDKRB2 Stable Cell Line-HEK293 is constructed by cloning the BDKRB2 gene into an expression vector and then introducing it into HEK293 cells through transfection. This process typically involves using viral vectors (such as lentivirus or adenovirus) to introduce the BDKRB2 gene into the cells, followed by selection and expansion to obtain a cell population stably expressing BDKRB2.
A: The Human BDKRB2 Stable Cell Line-HEK293 can be used for the development and screening of drugs targeting the BDKRB2 receptor, as well as for assessing the cytotoxicity of these drugs. By monitoring changes in BDKRB2 receptor activity, researchers can evaluate the potency and selectivity of drugs, while assessing drug safety through cell viability and proliferation.
A: The Human BDKRB2 Stable Cell Line-HEK293 provides a platform for studying the signaling pathways mediated by the BDKRB2 receptor. Through gene knockout or overexpression experiments in these cells, scientists can investigate the role of BDKRB2 in cellular signaling networks and its interactions with other signaling molecules.
A: The Human BDKRB2 Stable Cell Line-HEK293 can assist in identifying drug targets by conducting drug response experiments. For example, by comparing the activity of the BDKRB2 receptor in cells before and after drug treatment, it can be inferred whether the drug acts through the BDKRB2 receptor.
A: The Human BDKRB2 Stable Cell Line-HEK293 is of significant value in studying the different subtypes of the BDKRB2 receptor and their functional differences. By expressing different BDKRB2 subtypes in these cells, researchers can compare their differences in signal transduction and drug response.
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