Human BDKRB2 Stable Cell Line-HEK293

Name: Human BDKRB2 Stable Cell Line-HEK293
SKU: CSC-RG0007
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0007

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0007
Background	Bradykinin receptor B2 is a G-protein coupled receptor for Bradykinin, encoded by the BDKRB2 gene in humans. The B2 receptor is a G protein-coupled receptor that stimulates phospholipase C to increase intracellular free calcium. The 9 amino acid Bradykinin peptide elicits many responses including vasodilation, edema, smooth muscle spasm and pain fiber stimulation.
Target Gene	BDKRB2
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuBDKRB2
Alias	BDKRB2, B2R, BK-2, BK2, BKR2, BRB2
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	BDKRB2 bradykinin receptor B2 [ Homo sapiens ]
Gene Symbol	BDKRB2
Synonyms	B2R; BK2; BK-2; BKR2; BRB2
Gene Description	bradykinin receptor B2
GeneID	624
Uni ProtID	P30411
mRNA Refseq	NM_000623.3
Protein Refseq	NP_000614.1
Chromosome Location	14q32.1-q32.2
Function	bradykinin receptor activity; phosphatidylinositol phospholipase C activity; protease binding; protein binding; protein heterodimerization activity; type 1 angiotensin receptor binding;
Pathway	ACE Inhibitor Pathway, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem;
MIM	113503

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How is the Human BDKRB2 Stable Cell Line-HEK293 constructed through genetic engineering techniques?

A: The Human BDKRB2 Stable Cell Line-HEK293 is constructed by cloning the BDKRB2 gene into an expression vector and then introducing it into HEK293 cells through transfection. This process typically involves using viral vectors (such as lentivirus or adenovirus) to introduce the BDKRB2 gene into the cells, followed by selection and expansion to obtain a cell population stably expressing BDKRB2.

What applications does the Human BDKRB2 Stable Cell Line-HEK293 have in drug screening and toxicity studies?

A: The Human BDKRB2 Stable Cell Line-HEK293 can be used for the development and screening of drugs targeting the BDKRB2 receptor, as well as for assessing the cytotoxicity of these drugs. By monitoring changes in BDKRB2 receptor activity, researchers can evaluate the potency and selectivity of drugs, while assessing drug safety through cell viability and proliferation.

What role does the Human BDKRB2 Stable Cell Line-HEK293 play in studying the signaling pathways mediated by the BDKRB2 receptor?

A: The Human BDKRB2 Stable Cell Line-HEK293 provides a platform for studying the signaling pathways mediated by the BDKRB2 receptor. Through gene knockout or overexpression experiments in these cells, scientists can investigate the role of BDKRB2 in cellular signaling networks and its interactions with other signaling molecules.

How does the Human BDKRB2 Stable Cell Line-HEK293 assist in identifying drug targets during drug development?

A: The Human BDKRB2 Stable Cell Line-HEK293 can assist in identifying drug targets by conducting drug response experiments. For example, by comparing the activity of the BDKRB2 receptor in cells before and after drug treatment, it can be inferred whether the drug acts through the BDKRB2 receptor.

What is the value of the Human BDKRB2 Stable Cell Line-HEK293 in studying the subtype differences of the BDKRB2 receptor?

A: The Human BDKRB2 Stable Cell Line-HEK293 is of significant value in studying the different subtypes of the BDKRB2 receptor and their functional differences. By expressing different BDKRB2 subtypes in these cells, researchers can compare their differences in signal transduction and drug response.

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