Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RO01321
Host Cell : HUVECs Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RO01321 |
| Description | This cell line is engineered to stably express Homo sapiens (human) bradykinin receptor B2 (BDKRB2) in Human umbilical vein endothelial cells (HUVECs). GFP reporter gene is also expressed in this cell line allowing fluorescent tracking of cells. |
| Product Type | Human gene overexpression stable cell line |
| Target Gene | BDKRB2 |
| Gene Species | Homo sapiens (human) |
| Host Cell | HUVECs |
| Host Cell Species | Homo sapiens (Human) |
| Reporter | GFP |
| Applications |
1) investigation of gene function 2) screening and validation of antibodies |
| Size | One vial of frozen cells, typically >1x10^6cells/vial |
| Stability | This cell line is stable at least 10 passages. |
| Quality Control |
1) Real-time qPCR analysis of gene mRNA overexpression level 2) GFP fluorescent detection under fluorescent microscopy 3) mycoplasma detection |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Target Gene | BDKRB2 |
| Background | This gene encodes a receptor for bradykinin. The 9 aa bradykinin peptide elicits many responses including vasodilation, edema, smooth muscle spasm and pain fiber stimulation. Bradykinin is released upon activation by pathophysiologic conditions such as trauma and inflammation, and binds to its kinin receptors, B1 and B2. The B2 receptor associates with G proteins that stimulate a phosphatidylinositol-calcium second messenger system. [provided by RefSeq, Apr 2020] |
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The BDKRB2 stable expression in HUVECs from Creative Biogene is impressive. It is often difficult to maintain stable expression in endothelial cells without compromising their phenotype, but these cells are robust and perform beautifully in our bradykinin receptor signaling assays.
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