Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RG1801
Host Cell : HEK293T Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RG1801 |
| Description | This cell line is engineered to stably overexpress Human CCR5 in HEK293T cells. |
| Target Gene | CCR5 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Applications |
1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | CCR5 chemokine (C-C motif) receptor 5 (gene/pseudogene) [ Homo sapiens ] |
| Gene Symbol | CCR5 |
| Synonyms | CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5 |
| Gene Description | chemokine (C-C motif) receptor 5 |
| GeneID | 1234 |
| Uni ProtID | P51681 |
| mRNA Refseq | NM_000579.3 |
| Protein Refseq | NP_000570.1 |
| Chromosome Location | 3p21.31 |
| Function | C-C chemokine binding; C-C chemokine receptor activity; C-C chemokine receptor activity; actin binding; chemokine (C-C motif) ligand 5 binding; chemokine receptor activity; coreceptor activity; phosphatidylinositol phospholipase C activity; protein binding; |
| Pathway | Binding and entry of HIV virion, organism-specific biosystem; Chemokine receptors bind chemokines, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; |
| MIM | 601373 |
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We were pleased with the overall performance and usability of this CCR5 stable cell line. The HEK293T background was straightforward to maintain, and the stable-expression format supported consistent experimental planning. It has served as a useful cellular platform for receptor pharmacology, ligand characterization, and inhibitor screening.
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