Human CCR5-SNAP Stable Cell Line-HEK293

Name: Human CCR5-SNAP Stable Cell Line-HEK293
SKU: CSC-RG0042

Cat.No. : CSC-RG0042 Host Cell: HEK293

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Cell Line Information

Cell Culture Information

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Cat. No.	CSC-RG0042
Gene	CCR5
Gene Species	Homo sapiens (Human)
Alias	CCR5, CMKBR5, CD195, CKR 5, CKR5, CCR-5, CKR-5, CCCKR5, CMKBR5, CC-CKR-5
Host Cell	HEK293
Species	Homo sapiens (Human)
Morphology	Epithelial
Stability	Validated for at least 10 passages
Application	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Shipping	Dry ice
Storage	Liquid nitrogen

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. Two transcript variants encoding the same protein have been found for this gene

Customer Q&As

What is the significance of the Human CCR5-SNAP Stable Cell Line-HEK293 in HIV research?

A: The Human CCR5-SNAP Stable Cell Line-HEK293 is crucial for HIV research because CCR5 is a co-receptor used by HIV-1 for entry into host cells. The stable expression of CCR5 in HEK293 cells allows for the study of HIV-1 tropism, the development of antiretroviral drugs, and the investigation of host-pathogen interactions.

How does the SNAP tag in the Human CCR5-SNAP Stable Cell Line-HEK293 facilitate protein labeling?

A: The SNAP tag (Single Amino Acid Polymorphism) is an engineered protein tag that can be incorporated into proteins of interest. It enables the selective and covalent attachment of various molecules, such as fluorescent dyes or biotin, through a unique chemistry that does not occur in nature. This allows for the visualization and tracking of CCR5 in the HEK293 cells.

What are the advantages of using HEK293 cells for the Human CCR5-SNAP Stable Cell Line?

A: HEK293 cells are widely used for stable cell line generation due to their high transfection efficiency, rapid growth rate, and ease of genetic manipulation. They also have a relatively low background of endogenous CCR5 expression, which makes them suitable for studying the effects of exogenously expressed CCR5.

How can the Human CCR5-SNAP Stable Cell Line-HEK293 be utilized in drug screening for HIV?

A: This cell line can be used in high-throughput screening assays to identify compounds that inhibit HIV-1 entry by targeting CCR5. By monitoring the binding or internalization of labeled HIV-1 particles, researchers can assess the efficacy of potential antiretroviral drugs.

How does the Human CCR5-SNAP Stable Cell Line-HEK293 contribute to the understanding of CCR5's role in immune response?

A: By providing a controlled environment to study the function of CCR5, this cell line can help researchers understand its role in immune cell trafficking and its potential as a therapeutic target for diseases beyond HIV, such as cardiovascular diseases and certain cancers.

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