Robust fusion DNA polymerase

EMQZ1412

Robust fusion DNA polymerase has been genetically engineered to offer both high fidelity and robust performance, and thus can be used for multiple PCR applications. This enzyme is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate > 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase, Phusion is one of the most accurate thermostable polymerases available. Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products.

• PCR• Cloning• Long or Difficult Amplification• High-Throughput PCR

2,000 U/ml

50 ul Reaction System containing: 1× Robust fusion HF or GC Buffer, DNA template, 0.5 uM primer, 200 uM dNTPs (not supplied with enzyme), 3% DMSO (optional) and 1 U Robust fusion DNA polymerase.

100 U; 500 U

Purified from a recombinant E. coli strain that carraries Robust fusion DNA Polymerase gene.

Store at -20°C

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.