Robust fusion DNA polymerase

Name: Robust fusion DNA polymerase
SKU: EMQZ1412
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EMQZ1412

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Product Information

Cat. No.	EMQZ1412
Description	Robust fusion DNA polymerase has been genetically engineered to offer both high fidelity and robust performance, and thus can be used for multiple PCR applications. This enzyme is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate > 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase, Phusion is one of the most accurate thermostable polymerases available. Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products.
Source	Purified from a recombinant E. coli strain that carraries Robust fusion DNA Polymerase gene.
Concentration	2,000 U/ml
Applications	• PCR • Cloning • Long or Difficult Amplification • High-Throughput PCR
Size	100 U; 500 U
Unit Definition	One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
Reaction Conditions	50 ul Reaction System containing: 1× Robust fusion HF or GC Buffer, DNA template, 0.5 uM primer, 200 uM dNTPs (not supplied with enzyme), 3% DMSO (optional) and 1 U Robust fusion DNA polymerase.
Storage	Store at -20°C

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Customer Q&As

Does Robust fusion DNA polymerase have exonuclease activity?

A: Robust fusion DNA polymerase has 5′→3′ DNA polymerase activity and 3′→5′ exonuclease activity, producing blunt-ended PCR products.

Is the fidelity of the Robust fusion DNA polymerase high?

A: The synthesis rate of Robust fusion DNA polymerase is 2-4 kb/min, and its fidelity is 50 times higher than that of regular Taq DNA polymerase and 6 times higher than that of Pfu DNA polymerase.

What are the reaction conditions?

A: The 50 μl reaction system includes: 1× Robust fusion HF or GC Buffer, DNA template, 0.5 uM primers, 200 uM dNTPs (not provided with the enzyme), 3% DMSO (optional), and 1 U Robust fusion DNA polymerase.

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Customer Reviews

High fidelity

Robust fusion DNA polymerase is a DNA polymerase modified through protein engineering, possessing both rapid and high-fidelity characteristics, usable in various PCR reactions and performs well under complex template conditions.

United States

2022-11-03

High amplification efficiency

Robust fusion DNA polymerase can be used for long fragment amplification or difficult template amplification.

United States

2023-02-23

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Robust fusion DNA polymerase

Product Information

Q & A

Customer Reviews

Does Robust fusion DNA polymerase have exonuclease activity?

Is the fidelity of the Robust fusion DNA polymerase high?

What are the reaction conditions?

High fidelity

High amplification efficiency

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