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Taq Plus DNA Polymerase

For research use only. Not intended for any clinical use.
Cat.No.
EMDTS0012
Description
Taq Plus DNA Polymerase is provided by a mixture of Taq DNA Polymerase andthe proofreading enzyme Pfu DNA Polymerase. The product possesses boththe processivity of Taq and the high fidelity of Pfu. The specially formulated TaqPlus Polymerase allow amplification of the higher fidelity and longer templatesthan the single-enzyme formulations. It is also a better choice for amplifyingcomplex template, such as GC-rich template. It has a 5'→3' Exonuclease activityas well as a 3'→5' Exonuclease activity. Using Taq Plus DNA Polymerase DNAfragments up to 20 kb (up to 10 kb for GC-rich templates) could be amplified.
Note
For Research Use Only. Not for use in diagnostic procedures.
Quality Control
The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleasesconfirmed by appropriate quality tests. Functionally tested in PCR.
Size/Form
250U, 500U
Storage
Store at -20℃
Unit Definition
One unit is defined as the amount of the enzyme required to catalyze theincorporation of 10 nmoles of dNT P's into an acid-insoluble form in 30 minutes at74°C using hering sperm DNA as substrate.

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Q & A

Customer Reviews

Customer Q&As
How is 1U of the enzyme defined?

A: One unit is defined as the amount of the enzyme required to catalyze theincorporation of 10 nmoles of dNT P's into an acid-insoluble form in 30 minutes at74°C using hering sperm DNA as substrate.

What is the difference between Taq Plus DNA Polymerase and Taq DNA Polymerase?

A: Taq Plus DNA Polymerase is a mixture of Taq DNA Polymerase and proofreading enzyme Pfu DNA Polymerase, which combines the synthesis ability of Taq and the high fidelity of Pfu.

Can complex templates be amplified?

A: It is a better choice for amplifying complex templates, such as templates rich in GC content, with high fidelity.

Does the enzyme have exonuclease activity?

A: It has 5'→3' exonuclease activity and 3'→5' exonuclease activity.

How to store the product after receiving it?

A: After receiving the reagent, it can be stored directly at -20°C and placed on ice for use.

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Customer Reviews
Long fragment amplification

Taq Plus DNA Polymerase can amplify DNA fragments up to 20 kb, and up to 10 kb for templates rich in GC.

United States

03/15/2023

Complex templates

The fidelity of the enzyme is high and it can amplify complex templates. We have successfully amplified templates rich in GC using this enzyme.

United States

04/06/2023

High cost-effectiveness

The price is relatively cheap, and the quality of the product is good, with a high success rate of amplification.

United States

05/05/2023

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