Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : EMDTS0012
| Cat. No. | EMDTS0012 |
| Description | Taq Plus DNA Polymerase is provided by a mixture of Taq DNA Polymerase andthe proofreading enzyme Pfu DNA Polymerase. The product possesses boththe processivity of Taq and the high fidelity of Pfu. The specially formulated TaqPlus Polymerase allow amplification of the higher fidelity and longer templatesthan the single-enzyme formulations. It is also a better choice for amplifyingcomplex template, such as GC-rich template. It has a 5'→3' Exonuclease activityas well as a 3'→5' Exonuclease activity. Using Taq Plus DNA Polymerase DNAfragments up to 20 kb (up to 10 kb for GC-rich templates) could be amplified. |
| Size | 250U, 500U |
| Unit Definition | One unit is defined as the amount of the enzyme required to catalyze theincorporation of 10 nmoles of dNT P's into an acid-insoluble form in 30 minutes at74°C using hering sperm DNA as substrate. |
| Quality Control | The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleasesconfirmed by appropriate quality tests. Functionally tested in PCR. |
| Note | For Research Use Only. Not for use in diagnostic procedures. |
| Storage | Store at -20℃ |
A: One unit is defined as the amount of the enzyme required to catalyze theincorporation of 10 nmoles of dNT P's into an acid-insoluble form in 30 minutes at74°C using hering sperm DNA as substrate.
A: Taq Plus DNA Polymerase is a mixture of Taq DNA Polymerase and proofreading enzyme Pfu DNA Polymerase, which combines the synthesis ability of Taq and the high fidelity of Pfu.
A: It is a better choice for amplifying complex templates, such as templates rich in GC content, with high fidelity.
A: It has 5'→3' exonuclease activity and 3'→5' exonuclease activity.
A: After receiving the reagent, it can be stored directly at -20°C and placed on ice for use.
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Taq Plus DNA Polymerase can amplify DNA fragments up to 20 kb, and up to 10 kb for templates rich in GC.
The fidelity of the enzyme is high and it can amplify complex templates. We have successfully amplified templates rich in GC using this enzyme.
The price is relatively cheap, and the quality of the product is good, with a high success rate of amplification.
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