Cat.No. : CSC-RO0067 Host Cell: CHO-K1
| Cat. No. | CSC-RO0067 |
| Description | This cell line is a stably transfected cell line which expresses mouse cytotoxic T-lymphocyte-associated protein 4 (Ctla4) without any tag. |
| Background | Mice homozygous for a knock-out allele exhibit lethality at 3 to 4 weeks of age, decreased T cell numbers, abnormal T cell physiology, inflammation in mutliple organs, abnormal thymus morphology, and lymph node hypoplasia. |
| Gene | Ctla4 |
| Gene Species | Mus musculus (Mouse) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Target | Mouse |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Gene Name | Ctla4 cytotoxic T-lymphocyte-associated protein 4 [ Mus musculus (house mouse) ] |
| Gene Symbol | Ctla4 |
| Synonyms | Cd152; Ly-56; Ctla-4 |
| Gene ID | 12477 |
| UniProt ID | P09793 |
| Chromosome Location | 1 C2; 1 30.58 cM |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: This cell line was created by stably transfecting the Mus musculus CTLA4 gene into the CHO-K1 cell line.
A: This cell line stably expresses mouse CTLA4 on its surface, validated through FACS analysis.
A: When using human or animal-derived cell lines, one must adhere to biosafety and ethical regulations, including the assessment of risks associated with infectious pathogens.
A: Utilize cryopreservation techniques (like liquid nitrogen freezing) to ensure the cell characteristics remain unchanged.
A: Ensure purity and stability of the cell line during experiments and follow proper laboratory safety protocols.
A: Used to study the role of CTLA4 in modulating immune responses, aiding in the discovery and development of immunotherapies.
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