Mouse CTLA4 Stable Cell Line-CHO-K1

Name: Mouse CTLA4 Stable Cell Line-CHO-K1
SKU: CSC-RO0067
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0067

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0067
Description	This cell line is a stably transfected cell line which expresses mouse cytotoxic T-lymphocyte-associated protein 4 (Ctla4) without any tag.
Background	Mice homozygous for a knock-out allele exhibit lethality at 3 to 4 weeks of age, decreased T cell numbers, abnormal T cell physiology, inflammation in mutliple organs, abnormal thymus morphology, and lymph node hypoplasia.
Target	Mouse
Target Gene	Ctla4
Gene Species	Mus musculus (Mouse)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Source	CHO-K1
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Ctla4 cytotoxic T-lymphocyte-associated protein 4 [ Mus musculus (house mouse) ]
Gene Symbol	Ctla4
Synonyms	Cd152; Ly-56; Ctla-4
GeneID	12477
Uni ProtID	P09793
Chromosome Location	1 C2; 1 30.58 cM

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What is the origin of the Mouse CTLA4 Stable Cell Line-CHO?

A: This cell line was created by stably transfecting the Mus musculus CTLA4 gene into the CHO-K1 cell line.

What are the genotype and phenotype characteristics of this cell line?

A: This cell line stably expresses mouse CTLA4 on its surface, validated through FACS analysis.

What regulatory and ethical standards must be followed when using the Mouse CTLA4 Stable Cell Line-CHO?

A: When using human or animal-derived cell lines, one must adhere to biosafety and ethical regulations, including the assessment of risks associated with infectious pathogens.

How should the Mouse CTLA4 Stable Cell Line-CHO be properly preserved and resuscitated?

A: Utilize cryopreservation techniques (like liquid nitrogen freezing) to ensure the cell characteristics remain unchanged.

What considerations should be taken into account when using the Mouse CTLA4 Stable Cell Line-CHO in experiments?

A: Ensure purity and stability of the cell line during experiments and follow proper laboratory safety protocols.

What role does this cell line play in the discovery and development of immunotherapies?

A: Used to study the role of CTLA4 in modulating immune responses, aiding in the discovery and development of immunotherapies.

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