Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-2655 Host Cell : HEK293
| Cat. No. | CLOE-2655 |
| Introduction | This cell lysate overexpresses mouse Ctla4 (Met1-Phe162) with a C-terminal polyhistidine tag. Purification of target protein from this cell lysate has been verified. |
| Target Gene | Ctla4 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | Ctla4 cytotoxic T-lymphocyte-associated protein 4 [ Mus musculus (house mouse) ] |
| Gene Symbol | Ctla4 |
| Synonyms | Cd152; Ly-56; Ctla-4 |
| GeneID | 12477 |
| Uni ProtID | P09793 |
| Chromosome Location | 1 C2; 1 30.58 cM |
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