Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-0978 Host Cell : HEK293
| Cat. No. | CLOE-0978 |
| Introduction | This cell lysate over-expresses extracellular domain of human CTLA4 (NP_005205.2) (Met 1-Phe 162) fused with a polyhistidine tag at the C-terminus. Purification of target protein (s) from this cell lysate has been verified. |
| Target Gene | CTLA4 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | CTLA4 cytotoxic T-lymphocyte-associated protein 4 [ Homo sapiens ] |
| Gene Symbol | CTLA4 |
| Synonyms | CD; GSE; GRD4; ICOS; CD152; CTLA-4; IDDM12; CELIAC3 |
| GeneID | 1493 |
| Uni ProtID | P16410 |
| mRNA Refseq | NM_001037631.2 |
| Protein Refseq | NP_001032720.1 |
| Chromosome Location | 2q33 |
| Function | protein binding; |
| Pathway | Adaptive Immune System, organism-specific biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; CTLA4 inhibitory signaling, organism-specific biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; |
| MIM | 123890 |
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