Human TRPV6 Stable Cell Line-HEK293

Name: Human TRPV6 Stable Cell Line-HEK293
SKU: CSC-RI0204
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0204

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0204
Description	This cell line is engineered to overexpress human TRPV6.
Background	The human TRPV6 gene encodes the pore-forming subunit of TRPV6, a Ca2+-selective cation channel expressed in kidney and intestine epithelia.
Target Gene	TRPV6
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuTRPV6
Alias	TRPV6, CaT1, caT-L, CAT1, CATL, ZFAB, ECAC2, ABP/ZF, LP6728, HSA277909
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TRPV6 transient receptor potential cation channel, subfamily V, member 6 [ Homo sapiens ]
Gene Symbol	TRPV6
Synonyms	TRPV6; transient receptor potential cation channel, subfamily V, member 6; ECAC2, epithelial calcium channel 2; transient receptor potential cation channel subfamily V member 6; CaT1; caT-L; calcium channel CaT1; calcium transport protein 1; epithelial calcium channel 2; Alu-binding protein with zinc finger domain; epithelial apical membrane calcium transporter/channel CaT1; CAT1; CATL; ZFAB; ECAC2; ABP/ZF; LP6728; HSA277909;
GeneID	55503
Uni ProtID	Q9H1D0
mRNA Refseq	BC034814
Chromosome Location	7q34
Function	calcium channel activity; calmoduli
Pathway	Mineral absorption, organism-specific biosystem; Mineral absorption, conserved biosystem; Salivary secretion, organism-specific biosystem; Salivary secretion, conserved biosystem; Signaling events mediated by PTP1B, organism-specific biosystem; TCR signaling in naive CD4+ T cells, organism-specific biosystem; TCR signaling in naive CD8+ T cells, organism-specific biosystem;
MIM	606680

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