Human TRPC1 Stable Cell Line-HEK293

Name: Human TRPC1 Stable Cell Line-HEK293
SKU: CSC-RI0193
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0193

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0193
Description	This cell line is engineered to overexpress human TRPC1.
Background	The human TRPC1 gene encodes the pore-forming subunit of TRPC1, a non-selective, cation-permeable channel expressed in the CNS, vascular endothelium and smooth muscle, lymphocytes and epithelia. TRPC1 channels are therapeutic targets in pulmonary hypertension, vascular inflammation, heart failure, and keratosis.
Target Gene	TRPC1
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuTRPC1
Alias	TRPC1, HTRP 1, TRP-1, TRP1, HTRP-1, MGC133334, MGC133335
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TRPC1 transient receptor potential cation channel, subfamily C, member 1 [ Homo sapiens ]
Gene Symbol	TRPC1
Synonyms	TRP1; HTRP-1
Gene Description	transient receptor potential cation channel, subfamily C, member 1
GeneID	7220
Uni ProtID	P48995
mRNA Refseq	NM_003304.4
Protein Refseq	NP_003295.1
Chromosome Location	3q23
Function	calcium channel activity; cation channel activity; inositol 1,4,5 trisphosphate binding; ion channel binding; protein binding; store-operated calcium channel activity;
Pathway	Adaptive Immune System, organism-specific biosystem; Antigen Activates B Cell Receptor Leading to Generation of Second Messengers, organism-specific biosystem; Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Glutamatergic synapse, organism-specific biosystem; Glutamatergic synapse, conserved biosystem; Immune System, organism-specific biosystem;
MIM	602343

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