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Cat.No. : CSC-RI0205 Host Cell: CHO-K1
| Cat. No. | CSC-RI0205 |
| Description | This cell line is engineered to stably overexpress Human TRPV6 in CHO-K1 cells. |
| Gene | TRPV6 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Human TRPV6 Stable Cell Line - CHO-K1 is typically generated through stable transfection of CHO-K1 cells using an expression vector containing the human TRPV6 gene.
A: This cell line is primarily used for research related to the physiology and pharmacology processes associated with the TRPV6 channel, such as calcium ion transport, cell signaling, and cell proliferation.
A: Stability and expression levels are typically validated through methods such as immunoblotting for TRPV6 protein, real-time quantitative PCR, or functional cell assays.
A: This cell line can be used for screening and evaluating drugs, inhibitors, or activators related to the TRPV6 channel to study their pharmacological effects and potential applications.
A: Maintaining cell culture conditions, including appropriate media, temperature, CO2 concentration, and cell density, is crucial to ensure the stability and normal function of the cells.
A: The cellular characteristics and functionality of Human TRPV6 Stable Cell Line - CHO-K1 are typically influenced by maintaining stability, making the maintenance of cell stability crucial for research accuracy.
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