Human TRPV6 Stable Cell Line - CHO-K1

Name: Human TRPV6 Stable Cell Line - CHO-K1
SKU: CSC-RI0205
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0205

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0205
Description	This cell line is engineered to stably overexpress Human TRPV6 in CHO-K1 cells.
Target Gene	TRPV6
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TRPV6 transient receptor potential cation channel, subfamily V, member 6 [ Homo sapiens ]
Gene Symbol	TRPV6
Synonyms	TRPV6; transient receptor potential cation channel, subfamily V, member 6; ECAC2, epithelial calcium channel 2; transient receptor potential cation channel subfamily V member 6; CaT1; caT-L; calcium channel CaT1; calcium transport protein 1; epithelial calcium channel 2; Alu-binding protein with zinc finger domain; epithelial apical membrane calcium transporter/channel CaT1; CAT1; CATL; ZFAB; ECAC2; ABP/ZF; LP6728; HSA277909;
GeneID	55503
Uni ProtID	Q9H1D0
mRNA Refseq	BC034814
Chromosome Location	7q34
Function	calcium channel activity; calmoduli
Pathway	Mineral absorption, organism-specific biosystem; Mineral absorption, conserved biosystem; Salivary secretion, organism-specific biosystem; Salivary secretion, conserved biosystem; Signaling events mediated by PTP1B, organism-specific biosystem; TCR signaling in naive CD4+ T cells, organism-specific biosystem; TCR signaling in naive CD8+ T cells, organism-specific biosystem;
MIM	606680

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Q & A

Customer Reviews

Customer Q&As

What is the source of Human TRPV6 Stable Cell Line - CHO-K1, and how was this cell line generated?

A: Human TRPV6 Stable Cell Line - CHO-K1 is typically generated through stable transfection of CHO-K1 cells using an expression vector containing the human TRPV6 gene.

In which research fields and for what experimental purposes is Human TRPV6 Stable Cell Line - CHO-K1 primarily used?

A: This cell line is primarily used for research related to the physiology and pharmacology processes associated with the TRPV6 channel, such as calcium ion transport, cell signaling, and cell proliferation.

How is the stability and expression level of Human TRPV6 Stable Cell Line - CHO-K1 validated in research?

A: Stability and expression levels are typically validated through methods such as immunoblotting for TRPV6 protein, real-time quantitative PCR, or functional cell assays.

How is Human TRPV6 Stable Cell Line - CHO-K1 applied in drug screening and pharmacological research?

A: This cell line can be used for screening and evaluating drugs, inhibitors, or activators related to the TRPV6 channel to study their pharmacological effects and potential applications.

When conducting experiments with Human TRPV6 Stable Cell Line - CHO-K1, how are cell culture conditions controlled to maintain its stability?

A: Maintaining cell culture conditions, including appropriate media, temperature, CO2 concentration, and cell density, is crucial to ensure the stability and normal function of the cells.

Is the cellular characteristics and functionality of Human TRPV6 Stable Cell Line - CHO-K1 influenced by maintaining stability?

A: The cellular characteristics and functionality of Human TRPV6 Stable Cell Line - CHO-K1 are typically influenced by maintaining stability, making the maintenance of cell stability crucial for research accuracy.

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