Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RI0194
Host Cell : HEK293 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RI0194 |
| Description | This cell line is engineered to overexpress human TRPC6. |
| Background | The human TRPC6 gene encodes the pore-forming subunit of TRPC6, a non-selective, cation-permeable channel. TRPC6 channels are therapeutic targets in asthma, COPD, cancer, and pulmonary hypertension. |
| Target Gene | TRPC6 |
| Gene Species | Homo sapiens (Human) |
| Abbr | HEK293-HuTRPC6 |
| Alias | TRPC6, TRP6, TRP-6, FSGS2, FLJ11098, FLJ14863 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications |
1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Morphology | Epithelial |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | TRPC6 transient receptor potential cation channel, subfamily C, member 6 [ Homo sapiens ] |
| Gene Symbol | TRPC6 |
| Synonyms | TRP6; FSGS2 |
| GeneID | 7225 |
| Uni ProtID | Q9Y210 |
| mRNA Refseq | NM_004621.5 |
| Protein Refseq | NP_004612.2 |
| Chromosome Location | 11q22.1 |
| Function | inositol 1,4,5 trisphosphate binding; protein binding; store-operated calcium channel activity; |
| Pathway | Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; EPO signaling pathway, organism-specific biosystem; Effects of PIP2 hydrolysis, organism-specific biosystem; Elevation of cytosolic Ca2+ levels, organism-specific biosystem; Endothelins, organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; |
| MIM | 603652 |
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Switching to this TRPC6 HEK293 stable line vastly improved our assay window. The baseline noise is minimal, and the receptor expression levels are exceptionally high. The comprehensive QC data provided by Creative Biogene gave us great confidence right out of the box.
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