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Human TRPC6 Stable Cell Line-HEK293

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RI0194

Host Cell :   HEK293 Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RI0194
Description This cell line is engineered to overexpress human TRPC6.
Background The human TRPC6 gene encodes the pore-forming subunit of TRPC6, a non-selective, cation-permeable channel. TRPC6 channels are therapeutic targets in asthma, COPD, cancer, and pulmonary hypertension.
Target Gene TRPC6
Gene Species Homo sapiens (Human)
Abbr HEK293-HuTRPC6
Alias TRPC6, TRP6, TRP-6, FSGS2, FLJ11098, FLJ14863
Host Cell HEK293
Host Cell Species Homo sapiens (Human)
Applications

1. Gene expression studies

2. Signaling pathway research

3. Drug screening and toxicology

4. Channelopathies research

Size >1x106 frozen cells/vial
Stability Validated for at least 10 passages
Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium Complete medium supplemented with 10% (v/v) DMSO
Morphology Epithelial
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name TRPC6 transient receptor potential cation channel, subfamily C, member 6 [ Homo sapiens ]
Gene Symbol TRPC6
Synonyms TRP6; FSGS2
GeneID 7225
Uni ProtID Q9Y210
mRNA Refseq NM_004621.5
Protein Refseq NP_004612.2
Chromosome Location 11q22.1
Function inositol 1,4,5 trisphosphate binding; protein binding; store-operated calcium channel activity;
Pathway Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; EPO signaling pathway, organism-specific biosystem; Effects of PIP2 hydrolysis, organism-specific biosystem; Elevation of cytosolic Ca2+ levels, organism-specific biosystem; Endothelins, organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem;
MIM 603652
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Robust Expression

Switching to this TRPC6 HEK293 stable line vastly improved our assay window. The baseline noise is minimal, and the receptor expression levels are exceptionally high. The comprehensive QC data provided by Creative Biogene gave us great confidence right out of the box.

Italy

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