Cat.No. : CSC-RG0053 Host Cell: HEK293
| Cat. No. | CSC-RG0053 |
| Background | This gene belongs to a gene family of tachykinin receptors. These tachykinin receptors are characterized by interactions with G proteins and contain seven hydrophobic transmembrane regions. This gene encodes the receptor for the tachykinin substance P, also referred to as neurokinin 1. The encoded protein is also involved in the mediation of phosphatidylinositol metabolism of substance P. |
| Gene | TACR1 |
| Gene Species | Homo sapiens (Human) |
| Alias | TACR1, TAC1R, NK1R, SPR |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The establishment of the Human TACR1-SNAP Stable Cell Line-HEK293 is to provide a platform for studying the function of TACR1 (T cell activation receptor 1), especially its role in T cell activation processes. The SNAP tag (Succinimidyl 3-(2-benzoxazolyl-oxytris-pyrrolidino-phosphino-hexafluorophosphate) is a protein tag that allows various biochemical experiments to be performed in living cells, such as protein-protein interactions, protein localization, and protein modifications.
A: The expression of TACR1-SNAP can be precisely controlled by using an inducible promoter system, such as the tetracycline-inducible system. This system allows gene expression to be turned on when tetracycline or its derivatives are added and turned off in their absence, thus achieving strict control over TACR1-SNAP expression.
A: This cell line can be used for high-throughput screening (HTS) experiments to discover small molecule drugs that affect the function of TACR1. By monitoring changes in the activity of TACR1-SNAP, researchers can identify potential drug candidates that may influence the T cell activation pathway.
A: When performing immunofluorescence staining, it is necessary to ensure that the fixation and permeabilization steps do not disrupt the structure of the SNAP tag, and to choose a secondary antibody with fluorescent labeling that is compatible with the SNAP tag to avoid non-specific binding.
A: The interaction of TACR1-SNAP with other proteins can be studied through co-immunoprecipitation (co-IP) experiments. By using a SNAP tag-specific capture reagent, TACR1-SNAP and its interacting proteins can be pulled down, and these interacting proteins can then be identified through mass spectrometry.
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