Human TACR1-SNAP Stable Cell Line-HEK293

Name: Human TACR1-SNAP Stable Cell Line-HEK293
SKU: CSC-RG0053
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0053

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0053
Background	This gene belongs to a gene family of tachykinin receptors. These tachykinin receptors are characterized by interactions with G proteins and contain seven hydrophobic transmembrane regions. This gene encodes the receptor for the tachykinin substance P, also referred to as neurokinin 1. The encoded protein is also involved in the mediation of phosphatidylinositol metabolism of substance P.
Target Gene	TACR1
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuTACR1-SNAP
Alias	TACR1, TAC1R, NK1R, SPR
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TACR1 tachykinin receptor 1 [ Homo sapiens ]
Gene Symbol	TACR1
Synonyms	TACR1; tachykinin receptor 1; TAC1R; substance-P receptor; NK1R; NKIR; SPR; NK-1R; NK-1 receptor; tachykinin receptor 1 (substance P receptor; neurokinin-1 receptor);
GeneID	6869
Uni ProtID	P25103
Chromosome Location	2p13.1-p12
Function	G-protein coupled receptor activity; protein binding; receptor activity; signal transducer activity; substance P receptor activity; tachykinin receptor activity;
Pathway	Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Measles, organism-specific biosystem;
MIM	162323

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Q & A

Customer Reviews

Customer Q&As

What is the purpose of stably expressing Human TACR1-SNAP in the HEK293 cell line?

A: The establishment of the Human TACR1-SNAP Stable Cell Line-HEK293 is to provide a platform for studying the function of TACR1 (T cell activation receptor 1), especially its role in T cell activation processes. The SNAP tag (Succinimidyl 3-(2-benzoxazolyl-oxytris-pyrrolidino-phosphino-hexafluorophosphate) is a protein tag that allows various biochemical experiments to be performed in living cells, such as protein-protein interactions, protein localization, and protein modifications.

How can the expression level of TACR1-SNAP in the Human TACR1-SNAP Stable Cell Line-HEK293 be controlled?

A: The expression of TACR1-SNAP can be precisely controlled by using an inducible promoter system, such as the tetracycline-inducible system. This system allows gene expression to be turned on when tetracycline or its derivatives are added and turned off in their absence, thus achieving strict control over TACR1-SNAP expression.

What is the application of the Human TACR1-SNAP Stable Cell Line-HEK293 in drug screening?

A: This cell line can be used for high-throughput screening (HTS) experiments to discover small molecule drugs that affect the function of TACR1. By monitoring changes in the activity of TACR1-SNAP, researchers can identify potential drug candidates that may influence the T cell activation pathway.

What experimental details should be considered when performing immunofluorescence staining with the Human TACR1-SNAP Stable Cell Line-HEK293?

A: When performing immunofluorescence staining, it is necessary to ensure that the fixation and permeabilization steps do not disrupt the structure of the SNAP tag, and to choose a secondary antibody with fluorescent labeling that is compatible with the SNAP tag to avoid non-specific binding.

How can the interaction of TACR1 with other proteins be studied using the Human TACR1-SNAP Stable Cell Line-HEK293?

A: The interaction of TACR1-SNAP with other proteins can be studied through co-immunoprecipitation (co-IP) experiments. By using a SNAP tag-specific capture reagent, TACR1-SNAP and its interacting proteins can be pulled down, and these interacting proteins can then be identified through mass spectrometry.

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