Cat.No. : CSC-RG0017 Host Cell: HEK293
| Cat. No. | CSC-RG0017 |
| Background | Tachykinin receptor 1 (TACR1) also known as neurokinin 1 receptor (NK1R) or substance P receptor (SPR) belongs to a family of proteins characterized by the interaction with G proteins. TACR1 is localized both in the central nervous system (CNS) and peripheral tissues. Tachykinin receptor 1 presents great affinity for Substance P agonist. This receptor is an interesting drug target in the studies about analgesics and antidepressants. |
| Gene | TACR1 |
| Gene Species | Homo sapiens (Human) |
| Alias | TACR1, NK1R, NKIR, SPR, TAC1R |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Activation of the TACR1 receptor typically leads to an increase in intracellular calcium concentration. This can be measured and observed using calcium indicators like Fluo-4 AM.
A: Ligand-binding affinity for the TACR1 receptor can be assessed through radioligand binding assays or fluorescence polarization assays. These experiments can provide detailed information on ligand binding kinetics.
A: Activation of TACR1 can affect neurotransmitter release, which can be assessed by measuring the release of specific neurotransmitters like glutamate, GABA, etc.
A: Multiple signaling pathways, like the cAMP pathway, MAPK pathway, etc., may be activated or inhibited after TACR1 activation, which can be studied using specific pathway inhibitors or activators and subsequent molecular analyses.
A: Effective gene silencing can be achieved using siRNA specific to the TACR1 sequence, combined with an effective transfection method. Optimizing transfection conditions is crucial for enhancing silencing efficiency.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
