Human TACR1 Stable Cell Line-HEK293

Name: Human TACR1 Stable Cell Line-HEK293
SKU: CSC-RG0017
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0017

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0017
Background	Tachykinin receptor 1 (TACR1) also known as neurokinin 1 receptor (NK1R) or substance P receptor (SPR) belongs to a family of proteins characterized by the interaction with G proteins. TACR1 is localized both in the central nervous system (CNS) and peripheral tissues. Tachykinin receptor 1 presents great affinity for Substance P agonist. This receptor is an interesting drug target in the studies about analgesics and antidepressants.
Target Gene	TACR1
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuTACR1
Alias	TACR1, NK1R, NKIR, SPR, TAC1R
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TACR1 tachykinin receptor 1 [ Homo sapiens ]
Gene Symbol	TACR1
Synonyms	TACR1; tachykinin receptor 1; TAC1R; substance-P receptor; NK1R; NKIR; SPR; NK-1R; NK-1 receptor; tachykinin receptor 1 (substance P receptor; neurokinin-1 receptor);
GeneID	6869
Uni ProtID	P25103
Chromosome Location	2p13.1-p12
Function	G-protein coupled receptor activity; protein binding; receptor activity; signal transducer activity; substance P receptor activity; tachykinin receptor activity;
Pathway	Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Measles, organism-specific biosystem;
MIM	162323

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Customer Q&As

How does activation of the TACR1 receptor affect intracellular calcium concentration in Human TACR1 Stable Cell Line-HEK293?

A: Activation of the TACR1 receptor typically leads to an increase in intracellular calcium concentration. This can be measured and observed using calcium indicators like Fluo-4 AM.

How can the ligand-binding affinity of the TACR1 receptor be assessed in Human TACR1 Stable Cell Line-HEK293?

A: Ligand-binding affinity for the TACR1 receptor can be assessed through radioligand binding assays or fluorescence polarization assays. These experiments can provide detailed information on ligand binding kinetics.

What is the effect of Human TACR1 Stable Cell Line-HEK293 on neurotransmitter release?

A: Activation of TACR1 can affect neurotransmitter release, which can be assessed by measuring the release of specific neurotransmitters like glutamate, GABA, etc.

Which signaling pathways are activated or inhibited after TACR1 activation in Human TACR1 Stable Cell Line-HEK293?

A: Multiple signaling pathways, like the cAMP pathway, MAPK pathway, etc., may be activated or inhibited after TACR1 activation, which can be studied using specific pathway inhibitors or activators and subsequent molecular analyses.

How can siRNA-mediated gene silencing be most effectively performed in Human TACR1 Stable Cell Line-HEK293?

A: Effective gene silencing can be achieved using siRNA specific to the TACR1 sequence, combined with an effective transfection method. Optimizing transfection conditions is crucial for enhancing silencing efficiency.

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