Human GRIN1/GRIN2A Stable Cell Line-HEK293

Name: Human GRIN1/GRIN2A Stable Cell Line-HEK293
SKU: CSC-RI0174
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0174

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0174
Description	This cell line is engineered to overexpress human GRIN2A.
Background	The human GRIN1/GRIN2A genes encode the cationic-selective, n-methyl-d-aspartic acid receptor-channel expressed in the CNS. NMDA receptors are therapeutic targets in treatment of neurological disorders including seizure, pain, and Alzheimer"s disease.
Target Gene	GRIN2A
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuGRIN1/GRIN2A
Alias	GRIN2A, NMDAR2A, GluN2A, hNR2A, EPND, NR2A
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	GRIN2A glutamate receptor, ionotropic, N-methyl D-aspartate 2A [ Homo sapiens ]
Gene Symbol	GRIN2A
Synonyms	GRIN2A; glutamate receptor, ionotropic, N-methyl D-aspartate 2A; NMDAR2A; glutamate [NMDA] receptor subunit epsilon-1; GluN2A; hNR2A; NMDA receptor subtype 2A; N-methyl D-aspartate receptor subtype 2A; N-methyl-D-aspartate receptor subunit 2A; N-methyl-D-aspartate receptor channel, subunit epsilon-1; EPND; NR2A;
Gene ID	2903
Uni Prot ID	Q12879
m RNA Refseq	BC117131
Chromosome Location	16p13.2
Function	ATPase binding; N-methyl-D-aspartate selective glutamate receptor activity; calcium channel activity; cell adhesion molecule binding
Pathway	Activation of NMDA receptor upon glutamate binding and postsynaptic events, organism-specific biosystem; Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Amphetamine addiction, organism-specific biosystem; Amphetamine addiction, conserved biosystem; Amyotrophic lateral sclerosis (ALS), organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), conserved biosystem;
MIM	138253

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