Cat.No. : CSC-RI0016 Host Cell: HEK293
| Cat. No. | CSC-RI0016 |
| Description | This cell line is engineered to overexpress human GRIK2. |
| Background | GluR6 is a member of the ligand-gated, kainate family of glutamate receptors also known as GRIK2. It can form both hetero and homo-tetrameric, cation permeable, channels and is expressed at high levels in the hippocampus and striatum of the CNS. Pre- and postsynaptic kainate receptors are able to regulate both neurotransmitter release and excitability in a synapse-specific manner. GRIK2 has been implicated in a range of neurophysiological conditions such as autism, obsessive compulsive disorder, as well as disease states e.g. Schizophrenia, epilepsy and Huntingdon's disease. |
| Gene | GRIK2 |
| Gene Species | Homo sapiens (Human) |
| Alias | GRIK2, EAA4, GLR6, GLUK6, MGC74427 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The cell line was established by stably transfecting the human GRIK2 (glutamate receptor subtype 2) gene into HEK293 cells.
A: It is primarily used to study the role of GRIK2 in the nervous system, especially in neurotransmission and cognitive functions.
A: This cell line can be used for high-throughput screening of drugs affecting GRIK2 activity, particularly in the study of anti-epileptic and neurodegenerative disease medications.
A: It can be used to establish cell models of neurodegenerative diseases and psychiatric disorders, studying the pathological mechanisms of GRIK2.
A: It can be used to study the intracellular signaling pathways mediated by GRIK2, such as the regulation of calcium channel activity and neuronal excitability.
A: Operate under strict aseptic conditions, regularly replace the culture medium, and avoid cell overgrowth that can lead to degradation.
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