Human GRIK2 Stable Cell Line-HEK293

Name: Human GRIK2 Stable Cell Line-HEK293
SKU: CSC-RI0016
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0016

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0016
Description	This cell line is engineered to overexpress human GRIK2.
Background	GluR6 is a member of the ligand-gated, kainate family of glutamate receptors also known as GRIK2. It can form both hetero and homo-tetrameric, cation permeable, channels and is expressed at high levels in the hippocampus and striatum of the CNS. Pre- and postsynaptic kainate receptors are able to regulate both neurotransmitter release and excitability in a synapse-specific manner. GRIK2 has been implicated in a range of neurophysiological conditions such as autism, obsessive compulsive disorder, as well as disease states e.g. Schizophrenia, epilepsy and Huntingdon's disease.
Target Gene	GRIK2
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuGRIK2
Alias	GRIK2, EAA4, GLR6, GLUK6, MGC74427
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Homo sapiens glutamate receptor, ionotropic, kainate 2 DNA.
Gene Symbol	GRIK2
Synonyms	EAA4, GLR6, MRT6, GLUK6, GLUR6, GluK2

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Q & A

Customer Reviews

Customer Q&As

How was the Human GRIK2 Stable Cell Line-HEK293 established?

A: The cell line was established by stably transfecting the human GRIK2 (glutamate receptor subtype 2) gene into HEK293 cells.

What are the main uses of the Human GRIK2 Stable Cell Line-HEK293 in neuroscience research?

A: It is primarily used to study the role of GRIK2 in the nervous system, especially in neurotransmission and cognitive functions.

What are the advantages of using the Human GRIK2 Stable Cell Line-HEK293 in drug screening experiments?

A: This cell line can be used for high-throughput screening of drugs affecting GRIK2 activity, particularly in the study of anti-epileptic and neurodegenerative disease medications.

What are the applications of the Human GRIK2 Stable Cell Line-HEK293 in model studies?

A: It can be used to establish cell models of neurodegenerative diseases and psychiatric disorders, studying the pathological mechanisms of GRIK2.

What are the special uses of the Human GRIK2 Stable Cell Line-HEK293 in cell signaling research?

A: It can be used to study the intracellular signaling pathways mediated by GRIK2, such as the regulation of calcium channel activity and neuronal excitability.

How can cross-contamination and cell degradation be avoided when experimenting with the Human GRIK2 Stable Cell Line-HEK293?

A: Operate under strict aseptic conditions, regularly replace the culture medium, and avoid cell overgrowth that can lead to degradation.

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