Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RI0064
Host Cell : CHO Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RI0064 |
| Description | This cell line is engineered to overexpress human ASIC2. |
| Background | The human ACCN1 gene encodes the pore-forming subunit of ASIC2a, a proton-activated, Na+-permeable channel. ASIC2a channels widely expressed in sensory neurons of the peripheral and central nervous systems are therapeutic targets in pain and neurodegenerative diseases. |
| Target Gene | ASIC2 |
| Gene Species | Homo sapiens (Human) |
| Abbr | CHO-HuACCN1 |
| Alias | ASIC2, ACCN, ACCN1, ASIC2a, BNC1, BNaC1, MDEG, hBNaC1 |
| Host Cell | CHO |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Applications |
1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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