Human CHRNA2 Stable Cell Line-HEK293

Name: Human CHRNA2 Stable Cell Line-HEK293
SKU: CSC-RI0019
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0019

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0019
Description	This cell line is engineered to overexpress human CHRNA2.
Target Gene	CHRNA2
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuCHRNA2
Alias	CHRNA2
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CHRNA2 cholinergic receptor, nicotinic, alpha 2 (neuronal) [ Homo sapiens ]
Gene Symbol	CHRNA2
Gene Description	cholinergic receptor, nicotinic, alpha 2 (neuronal)
GeneID	1135
Uni ProtID	Q15822
mRNA Refseq	NM_000742.3
Protein Refseq	NP_000733.2
Chromosome Location	8p21
Function	acetylcholine receptor activity; acetylcholine-activated cation-selective channel activity;
Pathway	Acetylcholine Binding And Downstream Events, organism-specific biosystem; Activation of Nicotinic Acetylcholine Receptors, organism-specific biosystem; Highly calcium permeable nicotinic acetylcholine receptors, organism-specific biosystem; Highly calcium permeable postsynaptic nicotinic acetylcholine receptors, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem; Neuroactive ligand-receptor interaction, conserved biosystem; Neuronal System, organism-specific biosystem;
MIM	118502

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