Human P2RX1 Stable Cell Line-HEK293

Name: Human P2RX1 Stable Cell Line-HEK293
SKU: CSC-RI0180
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0180

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0180
Description	This cell line is engineered to overexpress human P2RX1.
Background	The human P2RX1 gene encodes P2X1, an ionotropic ATP receptor that functions as an ATP- or ADP-activated cation channel, expressed in smooth muscle, platelets, and sensory neurons. A P2RX1 mutation has been linked to a severe bleeding disorder arising from impaired ADP-induced platelet aggregation. P2X1 receptors are therapeutic targets in treatment of urinary incontinence and pain.
Target Gene	P2RX1
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuP2RX1
Alias	P2RX1, P2X1
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	P2RX1 purinergic receptor P2X, ligand-gated ion channel, 1 [ Homo sapiens ]
Gene Symbol	P2RX1
Synonyms	P2X1
Gene Description	purinergic receptor P2X, ligand-gated ion channel, 1
Gene ID	5023
Uni Prot ID	P51575
m RNA Refseq	NM_002558.2
Protein Refseq	NP_002549.1
Chromosome Location	17p13.3
Pathway	Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Elevation of cytosolic Ca2+ levels, organism-specific biosystem; Hemostasis, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem; Neuroactive ligand-receptor interaction, conserved biosystem; Platelet calcium homeostasis, organism-specific biosystem;
MIM	600845

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