Mouse Tnfrsf1b Stable Cell Line-HEK293T

Name: Mouse Tnfrsf1b Stable Cell Line-HEK293T
SKU: CSC-RO1064
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO1064

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO1064
Description	HEK293-Tnfrsf1b cell line is engineered to stably overexpress mouse Tnfrsf1b.
Target Gene	Tnfrsf1b
Gene Species	Mus musculus (Mouse)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Tnfrsf1b tumor necrosis factor receptor superfamily, member 1b [ Mus musculus ]
Gene Symbol	Tnfrsf1b
Synonyms	p75; TNFBR; Tnfr2; CD120b; TNF-R2; TNFR80; TNFRII; Tnfr-1; TNF-R75; TNF-R-II; TNF-alphaR2; TNFalpha-R2
Gene Description	tumor necrosis factor receptor superfamily, member 1b
GeneID	21938
Uni ProtID	P25119
mRNA Refseq	NM_011610.3
Protein Refseq	NP_035740.2
Chromosome Location	4 E1; 4 75.5 cM
Function	protein binding; tumor necrosis factor-activated receptor activity; tumor necrosis factor-activated receptor activity; ubiquitin protein ligase binding;
Pathway	Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Amyotrophic lateral sclerosis (ALS), organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), conserved biosystem; Apoptosis, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem;

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Customer Reviews

Customer Q&As

What are the advantages of the Mouse Tnfrsf1b Stable Cell Line-HEK293 cell line?

A: Mouse Tnfrsf1b Stable Cell Line-HEK293 overexpresses Mouse Tnfrsf1b in the HEK293 cell line. HEK293 cells rarely express the endogenous receptors needed for extracellular ligands and are easily transfected, making them a commonly used cell line for expressing exogenous genes.

What are the culture conditions for Mouse Tnfrsf1b Stable Cell Line-HEK293?

A: Using DMEM medium, normally 2% serum can maintain cell growth, and 10% can accelerate growth.

What are the passaging requirements for Mouse Tnfrsf1b Stable Cell Line-HEK293?

A: Mouse Tnfrsf1b Stable Cell Line-HEK293 cells change the liquid every 2-3 days. When the cells grow to 80%, they are passaged. 1:2 is recommended.

Has Mouse Tnfrsf1b Stable Cell Line-HEK293 undergone microbial and sterility testing?

A: All our products have undergone strict testing. If there are any problems upon receipt of the cells, please feedback promptly.

Are there any special considerations for Mouse Tnfrsf1b Stable Cell Line-HEK293?

A: Mouse Tnfrsf1b Stable Cell Line-HEK293 is an adherent cell. When passaging, trypsin is needed. To ensure the activity of trypsin, please remove the serum first.

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