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Cat.No. : CSC-RO1064 Host Cell: HEK293T
| Cat. No. | CSC-RO1064 |
| Description | HEK293-Tnfrsf1b cell line is engineered to stably overexpress mouse Tnfrsf1b. |
| Gene | Tnfrsf1b |
| Gene Species | Mus musculus (Mouse) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Mouse Tnfrsf1b Stable Cell Line-HEK293 overexpresses Mouse Tnfrsf1b in the HEK293 cell line. HEK293 cells rarely express the endogenous receptors needed for extracellular ligands and are easily transfected, making them a commonly used cell line for expressing exogenous genes.
A: Using DMEM medium, normally 2% serum can maintain cell growth, and 10% can accelerate growth.
A: Mouse Tnfrsf1b Stable Cell Line-HEK293 cells change the liquid every 2-3 days. When the cells grow to 80%, they are passaged. 1:2 is recommended.
A: All our products have undergone strict testing. If there are any problems upon receipt of the cells, please feedback promptly.
A: Mouse Tnfrsf1b Stable Cell Line-HEK293 is an adherent cell. When passaging, trypsin is needed. To ensure the activity of trypsin, please remove the serum first.
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