Human TNFRSF1B Stable Cell Line-HEK293T

Name: Human TNFRSF1B Stable Cell Line-HEK293T
SKU: CSC-RO1058
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO1058

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO1058
Description	HEK293-TNFRSF1B cell line is engineered to stably overexpress human TNFRSF1B.
Target Gene	TNFRSF1B
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TNFRSF1B tumor necrosis factor receptor superfamily, member 1B [ Homo sapiens ]
Gene Symbol	TNFRSF1B
Synonyms	p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II
Gene Description	tumor necrosis factor receptor superfamily, member 1B
GeneID	7133
Uni ProtID	P20333
mRNA Refseq	NM_001066.2
Protein Refseq	NP_001057.1
Chromosome Location	1p36.22
Function	protein binding; tumor necrosis factor-activated receptor activity; ubiquitin protein ligase binding;
Pathway	Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Amyotrophic lateral sclerosis (ALS), organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), conserved biosystem; Apoptosis, organism-specific biosystem; Apoptosis Modulation and Signaling, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem;
MIM	191191

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Q & A

Customer Reviews

Customer Q&As

What is the origin of the HEK293 cell line?

A: The HEK293 cell line was initially created by transforming human embryonic kidney cells with sheared adenovirus type 5 DNA. Since then, numerous subtypes and derivatives have been established, with HEK293, HEK293-T, and HEK293-F commonly used in biopharmaceutical production.

What are the applications of the HEK293 cell line in research?

A: The HEK293 cell line is extensively used for the production of recombinant proteins and viral vectors. It plays a crucial role in the production of adenoviral and adeno-associated viral vectors, due to the presence of adenoviral E1A/B genes which provide helper functions during viral vector production. Additionally, the HEK293 cell line is employed in the manufacture of retroviral vectors, as exemplified by the use of HEK293T cells.

What are the considerations when using the HEK293 cell line in research?

A: While the HEK293 cell line is highly effective for recombinant protein production, potential drawbacks include the use of xenogeneic genes as selection markers, which may lead to the high expression of non-human proteins in the HEK293 producer cell lines. Trace amounts of these proteins can contaminate the final product, potentially triggering allergic, adverse reactions, or immunogenicity.

How can protein yield and quality be optimized when producing proteins using the HEK293 cell line?

A: To enhance protein yield and quality, stable transfection of HEK293 cell lines can be employed. For instance, using modified HEK293S GnTI- (lacking N-acetylglucosaminyltransferase I) and Expi293F GnTI- suspension cells can facilitate the production of proteins with high yields and simpler glycosylation, which is beneficial for structural biology projects.

What are examples of therapeutic products produced using the HEK293 cell line for clinical applications?

A: The HEK293 cell line has been used to produce a variety of therapeutic proteins and viral vectors. For instance, during the COVID-19 pandemic, the rapid development of adenoviral vectored vaccines, including Ad5-nCOV and ChAdOX1-nCoV, was carried out in HEK293 cells.

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