Human TNFRSF1B Stable Cell Line-Jurkat

Name: Human TNFRSF1B Stable Cell Line-Jurkat
SKU: CSC-RO1059
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO1059

Host Cell : Jurkat Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO1059
Description	Jurkat-TNFRSF1B cell line is engineered to stably overexpress human TNFRSF1B.
Target Gene	TNFRSF1B
Gene Species	Homo sapiens (Human)
Host Cell	Jurkat
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TNFRSF1B tumor necrosis factor receptor superfamily, member 1B [ Homo sapiens ]
Gene Symbol	TNFRSF1B
Synonyms	p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II
Gene Description	tumor necrosis factor receptor superfamily, member 1B
GeneID	7133
Uni ProtID	P20333
mRNA Refseq	NM_001066.2
Protein Refseq	NP_001057.1
Chromosome Location	1p36.22
Function	protein binding; tumor necrosis factor-activated receptor activity; ubiquitin protein ligase binding;
Pathway	Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Amyotrophic lateral sclerosis (ALS), organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), conserved biosystem; Apoptosis, organism-specific biosystem; Apoptosis Modulation and Signaling, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem;
MIM	191191

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Q & A

Customer Reviews

Customer Q&As

What is the origin of the Human TNFRSF1B Stable Cell Line-Jurkat, and how was it established?

A: The Human TNFRSF1B Stable Cell Line-Jurkat originated from the Jurkat cell line, which is a well-established human T lymphocyte cell line. The stable cell line was developed by introducing and maintaining a stable expression of the TNFRSF1B gene in Jurkat cells through genetic modification techniques. This modification allows the cells to express the TNFRSF1B receptor on their surface consistently.

What are the primary applications of the Human TNFRSF1B Stable Cell Line-Jurkat in research and experimental studies?

A: The Human TNFRSF1B Stable Cell Line-Jurkat is primarily used in research related to the tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) and its signaling pathways. Common applications include studying apoptosis, immune responses, and inflammation regulation. Additionally, it can be utilized in drug screening and development studies targeting TNFRSF1B.

How can researchers maintain the stability of the Human TNFRSF1B Stable Cell Line-Jurkat in culture?

A: To maintain the stability of the Human TNFRSF1B Stable Cell Line-Jurkat, researchers should culture the cells under appropriate conditions. This includes maintaining the expression of TNFRSF1B by supplementing the culture medium with selection markers like antibiotics or other selection agents. Regular monitoring of the cell line's phenotype and gene expression is crucial to ensure stability over time.

What specific signaling pathways and downstream effects are typically studied using the Human TNFRSF1B Stable Cell Line-Jurkat?

A: Researchers commonly investigate the signaling pathways associated with TNFRSF1B, such as the NF-κB pathway, MAPK pathway, and JAK-STAT pathway, using the Human TNFRSF1B Stable Cell Line-Jurkat. Downstream effects of TNFRSF1B activation, including cytokine production, cell proliferation, and apoptosis, are also studied to understand the receptor's role in immune and inflammatory responses.

Are there any unique challenges or considerations when working with the Human TNFRSF1B Stable Cell Line-Jurkat compared to other Jurkat cell lines?

A: Yes, when working with the Human TNFRSF1B Stable Cell Line-Jurkat, researchers need to consider the potential effects of TNFRSF1B overexpression on cell behavior and signaling. It's essential to carefully design experiments and controls to account for these changes. Additionally, maintaining stable expression of TNFRSF1B may require ongoing selection pressure to prevent loss of expression.

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