Mouse CD40 Stable Cell Line - CHO-K1

CD40 is a 48 kDa type I transmembrane glycoprotein belonging to the tumor necrosis factor receptor (TNFR) superfamily. It is primarily expressed on antigen-presenting cells such as B cells, dendritic cells, and macrophages, and plays a central role in mediating humoral and cellular immune responses. Upon engagement with its ligand CD40L (CD154), CD40 triggers downstream signaling cascades including NF-κB, MAPK, and PI3K pathways, leading to B cell proliferation, antibody class switching, germinal center formation, and the activation of dendritic cells. The Mouse CD40 Stable Cell Line - CHO-K1 is generated by transfecting the full-length mouse CD40 gene into the CHO-K1 host cell line, followed by stable selection to achieve consistent and high-level surface expression of mouse CD40. This engineered cell line provides a homogeneous and reproducible system for studying CD40-mediated signaling in a controlled in vitro setting, free from the variability inherent in primary cell cultures.

This cell product is widely employed in immuno-oncology and drug discovery research. It serves as a key tool for evaluating the binding characteristics of anti-mouse CD40 antibodies, including agonist and antagonist candidates, as well as for screening small molecule modulators targeting the CD40 pathway. The cell line is compatible with flow cytometry, cell-based ELISA, and immunofluorescence assays for measuring antibody binding affinity and specificity. It can also be used in functional studies to assess CD40 downstream signaling activity, such as NF-κB reporter activation or cytokine release in co-culture systems. In addition, researchers leverage this stable cell line for CD40L binding assays, epitope mapping, and immunogenicity studies. The stable and uniform expression of mouse CD40 enables high-throughput screening and supports the preclinical evaluation of therapeutic antibodies and biologics aimed at modulating CD40-mediated immune responses in cancer, autoimmune diseases, and infectious diseases.