Mouse CD40 Stable Cell Line - CHO-K1

Name: Mouse CD40 Stable Cell Line - CHO-K1
SKU: CSC-RO0687
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0687

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0687
Description	This cell line is engineered to stably overexpress mouse CD40 antigen (CD40).
Target Gene	CD40
Gene Species	Mus musculus (Mouse)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Cd40 CD40 antigen [ Mus musculus ]
Gene Symbol	Cd40
Synonyms	IGM; p50; Bp50; GP39; IMD3; TRAP; HIGM1; T-BAM; Tnfrsf5; AI326936
Gene Description	CD40 antigen
GeneID	21939
Uni ProtID	P27512
mRNA Refseq	NM_170704.2
Protein Refseq	NP_733805.1
Chromosome Location	2 H3; 2 97.0 cM
Function	enzyme binding; protein binding; ubiquitin protein ligase binding;
Pathway	Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem;

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Q & A

Customer Reviews

Customer Q&As

What is the significance of using CHO-K1 cells for Mouse CD40 Stable Cell Line production, and what unique advantages do they offer?

A: Chinese hamster ovary (CHO) cells, specifically CHO-K1, are extensively utilized in biopharmaceuticals for producing monoclonal antibodies (mAbs), including immunoglobulin G (IgG). These cells are preferred due to their robust cell growth, effective post-translational modification capabilities, and adherence to good manufacturing practice (GMP) standards. CHO cells are key in achieving high and stable mAb production through genetic, transcriptional, and translational enhancements, as well as optimized culture conditions.

hat is the origin of the Mouse CD40 Stable Cell Line - CHO-K1, and how was it developed for research purposes?

A: The Mouse CD40 Stable Cell Line - CHO-K1 is a cell line derived from Chinese hamster ovary cells (CHO-K1). It was created through genetic engineering techniques where the CD40 gene from mice was introduced into CHO-K1 cells. This modification allows CHO-K1 cells to express the mouse CD40 protein on their surface, making them a valuable tool for studying CD40-related signaling pathways and immune responses in a mammalian system.

What are the primary applications of the Mouse CD40 Stable Cell Line - CHO-K1 in research and biotechnology?

A: The Mouse CD40 Stable Cell Line - CHO-K1 is primarily used in immunology and cell biology research. It serves as a model system to investigate various aspects of CD40-CD40L interactions, B cell activation, and immune responses. Researchers can use this cell line to study the downstream signaling pathways triggered by CD40 activation, screen for potential drug candidates targeting CD40, and assess the effects of different ligands or antibodies on CD40-mediated cellular responses.

Are there any specific precautions or considerations researchers should take when working with the Mouse CD40 Stable Cell Line - CHO-K1 in their experiments?

A: When using the Mouse CD40 Stable Cell Line - CHO-K1, researchers should be aware of the following considerations:Ensure appropriate containment and biosafety measures, as these cells have been genetically modified.<br/>Validate the stability of CD40 expression in the cell line over time to maintain experimental consistency.

Can you elaborate on the advantages of using the Mouse CD40 Stable Cell Line - CHO-K1 compared to other cell lines for CD40-related research?

A: The Mouse CD40 Stable Cell Line - CHO-K1 offers several advantages in CD40-related research: It allows for the study of CD40 signaling in a non-human mammalian system, which can provide insights into the conservation and divergence of CD40 functions.<br/>CHO-K1 cells are well-characterized and have a stable karyotype, making them a reliable model for consistent experimental results.

How does the Mouse CD40 Stable Cell Line - CHO-K1 contribute to our understanding of autoimmune diseases or cancer immunotherapy?

A: The Mouse CD40 Stable Cell Line - CHO-K1 contributes to autoimmune disease and cancer immunotherapy research in several ways: It can be employed to study the role of CD40 activation in the immune response against cancer cells, potentially leading to the development of novel immunotherapeutic strategies.<br/>By examining CD40-mediated B cell activation, researchers can gain insights into the pathogenesis of autoimmune diseases involving dysregulated immune responses.

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