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Cat.No. : CSC-RO1060 Host Cell: Jurkat
| Cat. No. | CSC-RO1060 |
| Description | Jurkat-CD40 cell line is engineered to stably overexpress human CD40. |
| Gene | CD40 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | Jurkat |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Jurkat grows faster.
A: Human CD40 Stable Cell Line-Jurkat is a suspension growth cell, which looks round and clear under normal growth conditions, resembling fish eggs.
A: Human CD40 Stable Cell Line-Jurkat is sensitive to mechanical force. During normal culture, avoid excessive force.
A: It is recommended to use high-quality fetal bovine serum. Differences in serum quality may cause changes in cell status.
A: After Jurkat cells are resuscitated, they usually take 3-5 days to recover. It is recommended not to operate within 48 hours after resuscitation.
A: Tests have shown that Jurkat's survival rate after resuscitation using non-programmed cryopreservatives is higher than that using programmed cryopreservatives. HyCyte? one-step cryopreservative is recommended.
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