Human CD40 Stable Cell Line-Jurkat

Name: Human CD40 Stable Cell Line-Jurkat
SKU: CSC-RO1060
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO1060

Host Cell : Jurkat Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO1060
Description	Jurkat-CD40 cell line is engineered to stably overexpress human CD40.
Target Gene	CD40
Gene Species	Homo sapiens (Human)
Host Cell	Jurkat
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CD40 CD40 molecule, TNF receptor superfamily member 5 [ Homo sapiens ]
Gene Symbol	CD40
Synonyms	p50; Bp50; CDW40; TNFRSF5
Gene Description	CD40 molecule, TNF receptor superfamily member 5
GeneID	958
Uni ProtID	P25942
mRNA Refseq	NM_152854.2
Protein Refseq	NP_690593.1
Chromosome Location	20q12-q13.2
Function	enzyme binding; protein binding; receptor activity; signal transducer activity; ubiquitin protein ligase binding;
Pathway	Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem;
MIM	109535

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

Why choose Jurkat over mouse T cells?

A: Jurkat grows faster.

What are the phenotypic features of Human CD40 Stable Cell Line-Jurkat?

A: Human CD40 Stable Cell Line-Jurkat is a suspension growth cell, which looks round and clear under normal growth conditions, resembling fish eggs.

What should be taken into account when culturing Human CD40 Stable Cell Line-Jurkat?

A: Human CD40 Stable Cell Line-Jurkat is sensitive to mechanical force. During normal culture, avoid excessive force.

Does Human CD40 Stable Cell Line-Jurkat have high culture condition requirements?

A: It is recommended to use high-quality fetal bovine serum. Differences in serum quality may cause changes in cell status.

How long after resuscitation can experiments be conducted on Human CD40 Stable Cell Line-Jurkat?

A: After Jurkat cells are resuscitated, they usually take 3-5 days to recover. It is recommended not to operate within 48 hours after resuscitation.

What cryopreservation medium should be used for Human CD40 Stable Cell Line-Jurkat?

A: Tests have shown that Jurkat's survival rate after resuscitation using non-programmed cryopreservatives is higher than that using programmed cryopreservatives. HyCyte? one-step cryopreservative is recommended.

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