Cat.No. : CSC-RO0044 Host Cell: CHO-K1
| Cat. No. | CSC-RO0044 |
| Description | This cell line is a stably transfected cell line which expresses Human CD40 without any tag. |
| Gene | CD40 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human CD40 Stable Cell Line-CHO offers several advantages for antibody production. Firstly, it is a mammalian cell line, which is known for its ability to perform complex post-translational modifications, leading to antibodies with high similarity to those produced in humans. Secondly, the stable expression of CD40 in CHO cells can facilitate the study of CD40 signaling pathways and its role in immune responses. Lastly, CHO cells are well-characterized and have a robust history in biotechnology, making them a reliable choice for large-scale production.
A: The Human CD40 Stable Cell Line-CHO provides a platform to study the activation and regulation of immune cells. CD40 is a critical co-stimulatory molecule involved in the activation of B cells and other immune cells. By using this cell line, researchers can investigate the downstream signaling pathways activated by CD40 ligation, which can lead to a better understanding of immune cell activation and the development of therapeutic strategies.
A: Maintaining and expanding the Human CD40 Stable Cell Line-CHO can be challenging due to the need for specific culture conditions to ensure stable expression of CD40. These cells may require selective pressure, such as antibiotic resistance markers, to maintain the CD40 expression. Additionally, the cells need to be regularly monitored for genetic stability and potential mutations that could affect CD40 expression or cell function.
A: The Human CD40 Stable Cell Line-CHO enables researchers to investigate the interactions between CD40 and CD40 ligand (CD40L), which is a critical aspect of immune cell communication. By expressing CD40 on the cell surface, this cell line allows for the study of CD40L binding, signaling, and the subsequent cellular responses, providing insights into the mechanisms of immune cell activation and regulation.
A: The Human CD40 Stable Cell Line-CHO can be utilized in vaccine development by producing antibodies that target CD40, which can enhance the immune response to a vaccine. Additionally, the cell line can be used to study the effects of CD40 activation on antigen-presenting cells, which can inform strategies for improving vaccine efficacy.
A: The Human CD40 Stable Cell Line-CHO provides a model system to study the role of CD40 signaling in B cell development and maturation. By manipulating the expression of CD40 and its ligands, researchers can investigate the impact of CD40 signaling on B cell proliferation, differentiation, and antibody production, which is crucial for understanding the adaptive immune response.
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