Human CD40 Stable Cell Line - HEK293T

Name: Human CD40 Stable Cell Line - HEK293T
SKU: CSC-RO0269
Rating: 4.0 (3 reviews)

Cat.No. : CSC-RO0269 Host Cell: HEK293T

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Cat. No.	CSC-RO0269
Description	HEK293T-CD40 is engineered to stably overexpress human CD40 molecule (CD40, NM_001250.5). Cell surface expression of human CD40 in this cell line has been analyzed by FACS.
Gene	CD40
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Stability	Validated for at least 10 passages
Application	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Shipping	Dry ice
Storage	Liquid nitrogen

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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CD40 is a costimulatory protein found on antigen-presenting cells and is critical for mediating multiple immune and inflammatory responses. It was first discovered in B cells and is a member of the tumor necrosis factor receptor superfamily. Binding of the CD40 protein ligand (CD40L) leads to numerous immune and inflammatory responses. The interaction between CD40 and its ligand (CD40L) is critical for the activation of B cells, dendritic cells, and macrophages, which in turn can lead to the production of specific antibodies against a variety of pathogens. In addition, due to its widespread expression and key role in immune activation and regulation, CD40 is considered a potential therapeutic target for a variety of diseases. Several CD40 antagonists have been developed and are in clinical trials for various autoimmune diseases and cancers.

Human CD40 Stable Cell Line - HEK293T is a cell line that stably expresses the human CD40 protein. These stable cell lines can become excellent tools for drug discovery and development, providing a reliable platform to study the function of CD40, study its role in various immune responses, and screen and test potential drugs targeting CD40.

The human CD40 stable cell line - HEK293T is widely used in biological research and drug discovery. Here are some of its applications:

Drug screening and discovery: Stable expression of human CD40 in HEK293T cells can be used to screen for potential therapeutic drugs that interact with CD40. This is particularly useful for studying various immune diseases and cancers.

Protein interaction studies: Human CD40 stable cell lines can also be used to study protein-protein interactions of CD40. Understanding these interactions is critical for discovering new drug targets or developing therapeutic strategies.

Biological research: In biological research, cell lines are useful in understanding the function and expression of CD40, its signaling pathways, and the consequences of its mutations. It could also advance research in immunology, cancer biology and several other fields.

Antibody production: Another important application is the production of monoclonal antibodies against the CD40 protein. These antibodies can be used for therapeutic purposes, particularly in the treatment of autoimmune diseases and cancer.

Vaccine development: Finally, human CD40 stable cell lines can be used to develop vaccines that modulate immune responses by targeting the CD40 protein.

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Customer Reviews

Excellent quality

The quality and performance of the Human CD40 Stable Cell Line - HEK293T exceeded our expectations. The cells are in good condition, with excellent health and growth rates. This product has proven to be very effective for our signal transduction studies related to CD40-mediated pathways.

Canada

11/06/2021

Simplified experimental design

Utilizing this stable cell line has greatly simplified our experimental design. The consistent expression of CD40 allows us to perform multiple assays without the variability that usually comes with transient transfections.

Canada

04/21/2021

Reliability

We have been using the Human CD40 Stable Cell Line - HEK293T for our immunology research. This cell line has maintained stable expression of CD40 over multiple passages and has become the core of our laboratory.

French

10/06/2023

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Human CD40 Stable Cell Line - HEK293T

Cell Line Information

Cell Culture Information

Safety and Packaging

Background

Applications

Publications

Q & A

Customer Reviews

Excellent quality

Simplified experimental design

Reliability

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