Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-1199 Host Cell : HEK293
| Cat. No. | CLOE-1199 |
| Introduction | This cell lysate over-expresses the extracellular domain of the human CD40 (NP_001241.1) (Met 1-Arg193) fused with a polyhistidine tag at the C-terminus. Purification of target protein (s) from this cell lysate has been verified. |
| Target Gene | CD40 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | CD40 CD40 molecule, TNF receptor superfamily member 5 [ Homo sapiens ] |
| Gene Symbol | CD40 |
| Synonyms | p50; Bp50; CDW40; TNFRSF5 |
| Gene Description | CD40 molecule, TNF receptor superfamily member 5 |
| GeneID | 958 |
| Uni ProtID | P25942 |
| mRNA Refseq | NM_152854.2 |
| Protein Refseq | NP_690593.1 |
| Chromosome Location | 20q12-q13.2 |
| Function | enzyme binding; protein binding; receptor activity; signal transducer activity; ubiquitin protein ligase binding; |
| Pathway | Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; |
| MIM | 109535 |
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