Human CD40 Stable Cell Line - A20

Name: Human CD40 Stable Cell Line - A20
SKU: CSC-RO1141
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO1141

Host Cell : A20 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO1141
Description	A20-CD40 cell line is engineered to stably overexpress human CD40.
Target Gene	CD40
Gene Species	Homo sapiens (Human)
Alias	Bp50, CDW40, TNFRSF5, p50
Host Cell	A20
Host Cell Species	Mus musculus (Mouse)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CD40 CD40 molecule, TNF receptor superfamily member 5 [ Homo sapiens ]
Gene Symbol	CD40
Synonyms	p50; Bp50; CDW40; TNFRSF5
Gene Description	CD40 molecule, TNF receptor superfamily member 5
GeneID	958
Uni ProtID	P25942
mRNA Refseq	NM_152854.2
Protein Refseq	NP_690593.1
Chromosome Location	20q12-q13.2
Function	enzyme binding; protein binding; receptor activity; signal transducer activity; ubiquitin protein ligase binding;
Pathway	Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem;
MIM	109535

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