S1 Nuclease

EMNT2030

Non-specific endonuclease, active primarily on single-stranded DNA and RNA

Catalyzes degradation of single-stranded nucleic acids into oligonucleotides and 5"-mononucleotides.Cleaves both single-stranded DNA and RNA, with stronger DNAse activity.Double-stranded DNA, double-stranded RNA and DNA-RNA hybrids are resistant to degradation at moderate enzyme concentration.Capable of cleavage of double-stranded nucleic acids at nicks, mismatches and small gaps.Relaxes/linearizes supercoiled plasmids.Removes protruding single-stranded ends.Used for S1 mapping of nucleic acids.Requires Zn2+ ions for activity.The enzyme is active up to 65 °C.

30 mM sodium acetate (pH 4.6).1 mM zinc acetate.50 mM NaCl.0.5 mg/ml activated DNA.5% glycerol.

Incubation at 37 °Cfor 10 minutes in a total reaction volume of 50 μl.

50000 Units;10000 Units

Aspergillus oryzae

20 mM Tris-HCl (pH 7.5 at 22 °C)50 mM NaCl0.1 mM ZnCl2 50% (v/v) glycerol