S1 Nuclease

Name: S1 Nuclease
SKU: EMNT2030
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EMNT2030

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Product Information

Cat. No.	EMNT2030
Description	Non-specific endonuclease, active primarily on single-stranded DNA and RNA
Source	Aspergillus oryzae
Applications	Catalyzes degradation of single-stranded nucleic acids into oligonucleotides and 5"-mononucleotides.Cleaves both single-stranded DNA and RNA, with stronger DNAse activity.Double-stranded DNA, double-stranded RNA and DNA-RNA hybrids are resistant to degradation at moderate enzyme concentration.Capable of cleavage of double-stranded nucleic acids at nicks, mismatches and small gaps.Relaxes/linearizes supercoiled plasmids.Removes protruding single-stranded ends.Used for S1 mapping of nucleic acids.Requires Zn2+ ions for activity.The enzyme is active up to 65 °C.
Size	50000 Units;10000 Units
Reaction Buffer	30 mM sodium acetate (pH 4.6).1 mM zinc acetate.50 mM NaCl.0.5 mg/ml activated DNA.5% glycerol.
Reaction Conditions	Incubation at 37 °Cfor 10 minutes in a total reaction volume of 50 μl.
Storage	20 mM Tris-HCl (pH 7.5 at 22 °C)50 mM NaCl0.1 mM ZnCl2 50% (v/v) glycerol

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Customer Q&As

How stable is the S1 Nuclease?

A: The S1 Nuclease is stable in 0.4 M NaCl, 0.6% SDS, and 0.8 M urea and requires a 10-fold increase in enzyme amount in the presence of 40-50% formaldehyde in the reaction mix.

How thermally stable is the S1 Nuclease?

A: S1 Nuclease is highly heat stable and remains active at 65-70°C in the presence of the substrate.

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