Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : EMQZ1413
| Cat. No. | EMQZ1413 |
| Description | RNase H (Ribonuclease H ) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. It does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA. |
| Source | E. coli strain |
| Concentration | 5,000 U/ml |
| Applications |
• Removal of mRNA prior to synthesis of second strand cDNA • RT-PCR and qRT-PCR: removal of RNA after first strand cDNA synthesis • Removal of the poly(A) sequences of mRNA after hybridization with oligo(dT) • Site-specific cleavage of RNA • Studies of in vitro polyadenylation reaction products |
| Size | 250 U; 1,250 U |
| Storage | Store at -20°C |
A: This product is sourced from E. coli H560 pol A1.
A: In addition to degrading RNA, this enzyme can also eliminate potential PCR error sources and increase the binding ability of primers in subsequent PCR processes.
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RNase H can specifically hydrolyze RNA hybridized with DNA but does not hydrolyze single-stranded DNA or double-stranded RNA.
After cDNA synthesis, using RNase H can increase the sensitivity of two-step RT-PCR analysis.
If oligo (dT) is present in the sequence, the addition of this reagent can remove the poly(A) sequence of mRNA.
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