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RNase H

Cat.No.
EMQZ1413
Description
RNase H (Ribonuclease H ) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. It does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA.
Applications
• Removal of mRNA prior to synthesis of second strand cDNA• RT-PCR and qRT-PCR: removal of RNA after first strand cDNA synthesis• Removal of the poly(A) sequences of mRNA after hybridization with oligo(dT)• Site-specific cleavage of RNA• Studies of in vitro polyadenylation reaction products
Concentration
5,000 U/ml
Size/Form
250 U; 1,250 U
Source
E. coli strain
Storage
Store at -20°C

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