RNase H

Name: RNase H
SKU: EMQZ1413
Rating: 4.0 (3 reviews)

For research use only. Not intended for any clinical use.

Cat.No.

EMQZ1413

Description

RNase H (Ribonuclease H ) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. It does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA.

Applications

• Removal of mRNA prior to synthesis of second strand cDNA• RT-PCR and qRT-PCR: removal of RNA after first strand cDNA synthesis• Removal of the poly(A) sequences of mRNA after hybridization with oligo(dT)• Site-specific cleavage of RNA• Studies of in vitro polyadenylation reaction products

Concentration

5,000 U/ml

Size/Form

250 U; 1,250 U

Source

E. coli strain

Storage

Store at -20°C

Publications

Q & A

Customer Reviews

Customer Q&As

Where does the enzyme come from?

A: This product is sourced from E. coli H560 pol A1.

What are the characteristics and advantages of this reagent?

A: In addition to degrading RNA, this enzyme can also eliminate potential PCR error sources and increase the binding ability of primers in subsequent PCR processes.

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Customer Reviews

High specificity

RNase H can specifically hydrolyze RNA hybridized with DNA but does not hydrolyze single-stranded DNA or double-stranded RNA.

United States

01/12/2020

Improves RT-PCR sensitivity

After cDNA synthesis, using RNase H can increase the sensitivity of two-step RT-PCR analysis.

United States

04/11/2021

Removes poly(A)

If oligo (dT) is present in the sequence, the addition of this reagent can remove the poly(A) sequence of mRNA.

United States

11/21/2021

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RNase H

Publications

Q & A

Customer Reviews

Where does the enzyme come from?

What are the characteristics and advantages of this reagent?

High specificity

Improves RT-PCR sensitivity

Removes poly(A)

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