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RNase H

For research use only. Not intended for any clinical use.

Cat. No. :   EMQZ1413

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Product Information

Cat. No. EMQZ1413
Description RNase H (Ribonuclease H ) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. It does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA.
Source E. coli strain
Concentration 5,000 U/ml
Applications • Removal of mRNA prior to synthesis of second strand cDNA
• RT-PCR and qRT-PCR: removal of RNA after first strand cDNA synthesis
• Removal of the poly(A) sequences of mRNA after hybridization with oligo(dT)
• Site-specific cleavage of RNA
• Studies of in vitro polyadenylation reaction products
Size 250 U; 1,250 U
Storage Store at -20°C
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Customer Q&As
Where does the enzyme come from?

A: This product is sourced from E. coli H560 pol A1.

What are the characteristics and advantages of this reagent?

A: In addition to degrading RNA, this enzyme can also eliminate potential PCR error sources and increase the binding ability of primers in subsequent PCR processes.

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Customer Reviews
High specificity

RNase H can specifically hydrolyze RNA hybridized with DNA but does not hydrolyze single-stranded DNA or double-stranded RNA.

United States

Improves RT-PCR sensitivity

After cDNA synthesis, using RNase H can increase the sensitivity of two-step RT-PCR analysis.

United States

Removes poly(A)

If oligo (dT) is present in the sequence, the addition of this reagent can remove the poly(A) sequence of mRNA.

United States

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