RecBCD Nuclease

Name: RecBCD Nuclease
SKU: EMNT2019
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EMNT2019

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Product Information

Cat. No.	EMNT2019
Description	RecBCD Nuclease is an exonuclease from E. coli that degrades ssDNA and dsDNA. Hydrolysis of the DNA is bidirectional from both the 3′ and 5′ ends and is processive, producing nucleoside monophosphates. Magnesium is required for the exonuclease activity, while calcium, nickel, zinc, and copper inhibit exonuclease activity. Calcium allows dsDNA unwinding (helicase activity) without hydrolysis.
Concentration	10000 units/ml
Applications	Removal of contaminating bacterial chromosomal DNA in plasmid, fosmid, cosmid, and BAC clone or vector preparations.
Size	1000 units
Unit Definition	One unit of RecBCD Nuclease degrades 1 nmol of deoxyribonucleotides in linear dsDNA in 30 minutes at 37 °C in 1X Reaction Buffer containing 1 mM ATP.
Storage	50% glycerol solution containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.

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Customer Q&As

Is RecBCD effective in other NEB buffers?

A: Yes, RecBCD Nuclease exhibits similar activity in NEB buffers 2, 3, and 4 (with the addition of 1 mM ATP).

What are the characteristics of the RecBCD Nuclease?

A: RecBCD Nuclease degrades linear ssDNA and dsDNA while preserving gapped and supercoiled plasmid DNA.

In the plasmid preparation process, what is the best exonuclease to remove chromosomal DNA?

A: Exonuclease V (RecBCD) can remove residual chromosomal DNA after plasmid purification.

How is 1U of the RecBCD Nuclease defined?

A: The definition of 1 unit of the RecBCD Nuclease is as follows: Under the condition of 37°C and a total reaction volume of 50 μl, the amount of enzyme required to generate 1 nmol of acid-soluble deoxyribonucleotides from double-stranded DNA within 30 minutes.

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