RecBCD Nuclease is an exonuclease from E. coli that degrades ssDNA and dsDNA. Hydrolysis of the DNA is bidirectional from both the 3′ and 5′ ends and is processive, producing nucleoside monophosphates. Magnesium is required for the exonuclease activity, while calcium, nickel, zinc, and copper inhibit exonuclease activity. Calcium allows dsDNA unwinding (helicase activity) without hydrolysis.
Removal of contaminating bacterial chromosomal DNA in plasmid, fosmid, cosmid, and BAC clone or vector preparations.
One unit of RecBCD Nuclease degrades 1 nmol of deoxyribonucleotides in linear dsDNA in 30 minutes at 37 °C in 1X Reaction Buffer containing 1 mM ATP.
50% glycerol solution containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.