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RecBCD Nuclease

Cat.No.
EMNT2019
Description
RecBCD Nuclease is an exonuclease from E. coli that degrades ssDNA and dsDNA. Hydrolysis of the DNA is bidirectional from both the 3′ and 5′ ends and is processive, producing nucleoside monophosphates. Magnesium is required for the exonuclease activity, while calcium, nickel, zinc, and copper inhibit exonuclease activity. Calcium allows dsDNA unwinding (helicase activity) without hydrolysis.
Applications
Removal of contaminating bacterial chromosomal DNA in plasmid, fosmid, cosmid, and BAC clone or vector preparations.
Unit Definition
One unit of RecBCD Nuclease degrades 1 nmol of deoxyribonucleotides in linear dsDNA in 30 minutes at 37 °C in 1X Reaction Buffer containing 1 mM ATP.
Concentration
10000 units/ml
Size
1000 units
Storage Buffer
50% glycerol solution containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.
Storage Conditions
store at -20 °C.
Related Products
Cat#
Product Name
Recognition Site
Size
Price
EMNT2030
N/A
50000 Units;10000 Units


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USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-biogene.com

Europe
Tel: 44-207-097-1828
Email: info@creative-biogene.com
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CONTACT CREATIVE BIOGENE

45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-biogene.com

Europe
Tel: 44-207-097-1828

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