Lambda Terminase

Name: Lambda Terminase
SKU: EMNT2013

For research use only. Not intended for any clinical use.

Cat.No.

EMNT2013

Description

Lambda Terminase is an endonuclease encoded by bacteriophage lambda that recognizes and cleaves DNA at cos sites, generating 5′ overhangs of 12 bases in length.3 Since the 12-base cos site sequence is rare, the sizes of inserts in BAC, fosmid, or cosmid clones can be rapidly determined.4 Epicentre"s Lambda Terminase is highly purified to provide reproducible cleavage with minimum nonspecific nicking activity. A typical digestion requires only 1 unit of Lambda Terminase for each microgram of DNA.

Applications

Rapid sizing of BAC, fosmid, or cosmid clones. Generation of restriction maps of BAC, cosmid, or fosmid clone inserts.1 Linearization of cos site-containing clones for in vitro packaging. Specific cleavage of chromosomal DNA for physical mapping.2

Concentration

2000 units/ml

Size/Form

50 units; 200 units

Storage

50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.

Unit Definition

One unit of Lambda Terminase linearizes up to 70% of 1 μg of the cosmid DNA vector pHC79 in 60 minutes at room temperature under standard assay conditions.

Publications

Q & A

Customer Reviews

Customer Q&As

How is the 5' end cleavage speed when using the Lambda Terminase?

A: The Lambda Terminase cutting rate at the 5' hydroxyl end is 20 times slower than at the 5' phosphate end.

How is the activity of the Lambda Terminase in various NEB buffers?

A: Lambda exonuclease activity in different buffers is as follows: NEBuffer 1: 15%; NEBuffer 2: 40%; NEBuffer 3: 10%; NEBuffer 4: 40%; CutSmart: 50%; Nuclease I Reaction Buffer: 100%; Thermopol Reaction Buffer: 100%.

What are the differences between T5 and Lambda exonucleases?

A: Unlike Lambda exonuclease, T5 exonuclease can degrade gapped circular dsDNA. T5 exonuclease can also act on ssDNA, which Lambda exonuclease cannot.

Is the cleavage speed the same for single-stranded and double-stranded DNA?

A: No, the Lambda Terminase cutting rate for single-stranded DNA is 100 times slower than for double-stranded DNA.

Can Lambda Restriction Enzyme function in rCutSmart Buffer?

A: Lambda Restriction Enzyme can function in rCutSmart Buffer. To assess its functional activity percentage in rCutSmart, as well as the activity percentages of other DNA modifying enzymes in the cloning workflow, please refer to the following data.

What is the reaction buffer?

A: Reaction buffer system: 67 mM Glycine-KOH, 2.5 mM MgCl2, 50 μg/ml BSA.

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