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Lambda Terminase

Cat.No.
EMNT2013
Description
Lambda Terminase is an endonuclease encoded by bacteriophage lambda that recognizes and cleaves DNA at cos sites, generating 5′ overhangs of 12 bases in length.3 Since the 12-base cos site sequence is rare, the sizes of inserts in BAC, fosmid, or cosmid clones can be rapidly determined.4 Epicentre"s Lambda Terminase is highly purified to provide reproducible cleavage with minimum nonspecific nicking activity. A typical digestion requires only 1 unit of Lambda Terminase for each microgram of DNA.
Applications
Rapid sizing of BAC, fosmid, or cosmid clones. Generation of restriction maps of BAC, cosmid, or fosmid clone inserts.1 Linearization of cos site-containing clones for in vitro packaging. Specific cleavage of chromosomal DNA for physical mapping.2
Unit Definition
One unit of Lambda Terminase linearizes up to 70% of 1 μg of the cosmid DNA vector pHC79 in 60 minutes at room temperature under standard assay conditions.
Concentration
2000 units/ml
Size
50 units; 200 units
Storage Buffer
50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.
Related Products
Cat#
Product Name
Recognition Site
Size
Price
EMNT2021
N/A
10 mg


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USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-biogene.com

Europe
Tel: 44-207-097-1828
Email: info@creative-biogene.com
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CONTACT CREATIVE BIOGENE

45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-biogene.com

Europe
Tel: 44-207-097-1828

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