Lambda Terminase is an endonuclease encoded by bacteriophage lambda that recognizes and cleaves DNA at cos sites, generating 5′ overhangs of 12 bases in length.3 Since the 12-base cos site sequence is rare, the sizes of inserts in BAC, fosmid, or cosmid clones can be rapidly determined.4 Epicentre"s Lambda Terminase is highly purified to provide reproducible cleavage with minimum nonspecific nicking activity. A typical digestion requires only 1 unit of Lambda Terminase for each microgram of DNA.
Rapid sizing of BAC, fosmid, or cosmid clones. Generation of restriction maps of BAC, cosmid, or fosmid clone inserts.1 Linearization of cos site-containing clones for in vitro packaging. Specific cleavage of chromosomal DNA for physical mapping.2
One unit of Lambda Terminase linearizes up to 70% of 1 μg of the cosmid DNA vector pHC79 in 60 minutes at room temperature under standard assay conditions.
50 units; 200 units
50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.