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RNase T1

For research use only. Not intended for any clinical use.

Cat. No. :   EMNT2029

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Product Information

Cat. No. EMNT2029
Description Ribonuclease T1 (RNase T1) is an endoribonuclease that specifically cleaves RNA or deaminated RNA at the 3′ end of guanosine residues and adjacent nucleotides through a 2′,3′ cyclic phosphate intermediate mechanism. Epicentre"s RNase T1 is cloned from Aspergillus oryzae and overexpressed in E. coli to produce a highly pure enzyme without contaminating DNase or nonspecific RNase activity.
Concentration 1000000 units/ml
Applications RNA mapping and structure studies. Removal of RNA from DNA preparations. RNA protection assays.
Size 100000 Units
Unit Definition One unit of RNase T1 converts 100 ng of E. coli ribosomal RNA into acid-soluble nucleotides in 5 seconds at 37 °C under standard assay conditions.
Storage 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, and 0.1 mM EDTA.
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Customer Q&As
In which experiments is the RNase T1 commonly used?

A: It can be used for 1) RNA mapping and structure studies, 2) Removal of RNA from DNA preparations, and 3) RNA protection assays.

How is RNase T1 activity defined?

A: One unit of RNase T1 converts 100 ng of E. coli ribosomal RNA into acid-soluble nucleotides in 5 seconds at 37 °C under standard assay conditions.

Where does the RNase T1 originate from?

A: RNase T1 is cloned from Aspergillus oryzae and overexpressed in E. coli.

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Customer Reviews
High enzyme activity

The activity of the enzyme reaches 1000000 units/ml, indicating high activity.

Germany

No contamination

This product is a highly pure enzyme and does not contain contaminating DNase or non-specific RNase activity.

United States

Ribonuclease protection assay

In ribonuclease protection assays, RNase T1 can be used in combination with RNase A.

United States

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