Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : EMNT2029
| Cat. No. | EMNT2029 |
| Description | Ribonuclease T1 (RNase T1) is an endoribonuclease that specifically cleaves RNA or deaminated RNA at the 3′ end of guanosine residues and adjacent nucleotides through a 2′,3′ cyclic phosphate intermediate mechanism. Epicentre"s RNase T1 is cloned from Aspergillus oryzae and overexpressed in E. coli to produce a highly pure enzyme without contaminating DNase or nonspecific RNase activity. |
| Concentration | 1000000 units/ml |
| Applications | RNA mapping and structure studies. Removal of RNA from DNA preparations. RNA protection assays. |
| Size | 100000 Units |
| Unit Definition | One unit of RNase T1 converts 100 ng of E. coli ribosomal RNA into acid-soluble nucleotides in 5 seconds at 37 °C under standard assay conditions. |
| Storage | 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, and 0.1 mM EDTA. |
A: It can be used for 1) RNA mapping and structure studies, 2) Removal of RNA from DNA preparations, and 3) RNA protection assays.
A: One unit of RNase T1 converts 100 ng of E. coli ribosomal RNA into acid-soluble nucleotides in 5 seconds at 37 °C under standard assay conditions.
A: RNase T1 is cloned from Aspergillus oryzae and overexpressed in E. coli.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The activity of the enzyme reaches 1000000 units/ml, indicating high activity.
This product is a highly pure enzyme and does not contain contaminating DNase or non-specific RNase activity.
In ribonuclease protection assays, RNase T1 can be used in combination with RNase A.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.