RNase III

Name: RNase III
SKU: EMNT2026

For research use only. Not intended for any clinical use.

Cat.No.

EMNT2026

Description

Ribonuclease III (RNase III) from E. coli is an endoribonuclease that specifically digests dsRNA to dsRNA fragments that have two-base, 3′ overhangs.5,6 Complete digestion of dsRNA results in dsRNA fragments of 12-15 bp.RNase III can digest dsRNA composed of canonical ribonucleotides as well as DuraScript® RNA produced using Epicentre"s DuraScribe® T7 Transcription Kit, which contains 2′-fluorine-CMP and 2′-fluorine-UMP in place of the canonical CMP and UMP nucleotides.

Applications

Digestion of long dsRNA to short dsRNA. RNA structure studies.1 RNA processing and maturation studies.

Concentration

1000 units/ml

Size/Form

50 units

Storage

50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 0.5 M NaCl, and 0.1% Triton® X-100.

Unit Definition

One unit of RNase III, E. coli, solubilizes 1 nmol of ribonucleotide in 30 minutes at 37 °C using poly(A)-poly(U) as the substrate under standard assay conditions.

Publications

Q & A

Customer Reviews

Customer Q&As

What is the function of RNase III?

A: In Escherichia coli, RNase III cleaves ribosomal RNA (rRNA) precursors during rRNA maturation.

What are the products after RNase III cleavage, and how do they differ from those generated by Dicer?

A: RNase III processes double-stranded RNA (dsRNA) into 12-15 bp dsRNA fragments with 2-3 nucleotide 3' overhangs, 5' phosphates, and 3' hydroxyl ends. Therefore, the ends and overhangs of RNase III cleavage products are similar to those generated by Dicer in the eukaryotic RNAi pathway.

Is RNase III an endonuclease or exonuclease?

A: RNase III is a double-stranded RNA (dsRNA)-specific exoribonuclease.

How is one unit of RNase III defined?

A: One unit is defined as the amount of enzyme required to cleave 1 μg of 500 bp dsRNA substrate into approximately 12-30 bp fragments within 60 minutes at 37°C.

What are the specifications for RNase III?

A: RNase III is available in a 50-unit size.

Can RNase III be stored at -80°C, and how long can it be stored?

A: RNase III can be stored at -80°C, but typically, RNase III and the reaction buffer should be stored at -20°C and can be kept for up to one year.

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