Ribonuclease III (RNase III) from E. coli is an endoribonuclease that specifically digests dsRNA to dsRNA fragments that have two-base, 3′ overhangs.5,6 Complete digestion of dsRNA results in dsRNA fragments of 12-15 bp.RNase III can digest dsRNA composed of canonical ribonucleotides as well as DuraScript® RNA produced using Epicentre"s DuraScribe® T7 Transcription Kit, which contains 2′-fluorine-CMP and 2′-fluorine-UMP in place of the canonical CMP and UMP nucleotides.
Digestion of long dsRNA to short dsRNA. RNA structure studies.1 RNA processing and maturation studies.
One unit of RNase III, E. coli, solubilizes 1 nmol of ribonucleotide in 30 minutes at 37 °C using poly(A)-poly(U) as the substrate under standard assay conditions.
50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 0.5 M NaCl, and 0.1% Triton® X-100.