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RNase I

For research use only. Not intended for any clinical use.
Cat.No.
EMQZ1414
Description
Ribonuclease I (RNase I), an endoribonuclease, preferentially hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates via nucleoside 2', 3'-cyclic monophosphate intermediates
Applications
• Removal of RNA from DNA solutions• Removal of RNA from recombinant protein preparations• Ribonuclease protection assays
Concentration
10,000 U/ml
Size/Form
1,000 U; 5,000 U
Source
E. coli strain
Storage
Store at -20°C

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Q & A

Customer Reviews

Customer Q&As
What kind of experiments are these products generally used for?

A: This product can be used for RNA removal from DNA solutions, RNA removal from recombinant protein preparations, ribonuclease protection assays, etc.

Does this product inhibit the activity of mammalian cells during cell experiments?

A: No, RNase inhibitors from mammalian cells do not affect the activity of RNase I.

Do you have any suggestions for diluting the reagent to a working solution?

A: When working with a dilution solution, Triton X-100 or BSA (0.1 mg/mL) can prevent RNase I from adhering to glass containers.

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Customer Reviews
Stable activity

It is stable and active under various reaction conditions and does not require metal ions to generate activity.

United States

01/02/2021

Strong specificity

In experiments, the enzyme only degrades RNA and does not affect the quality of proteins or DNA.

United States

03/25/2022

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