RNase A

Name: RNase A
SKU: EMNT2021
Rating: 4.0 (2 reviews)

For research use only. Not intended for any clinical use.

Cat.No.

EMNT2021

Description

Ribonuclease A (RNase A) is an endoribonuclease that cleaves single-stranded RNA at the 3′ end of pyrimidine residues, forming oligoribonucleotides having 3′-terminal pyrimidine-3′-phosphates. Pyrimidine-3′-monophosphates are also released by RNase A cleavage of adjacent pyrimidine nucleotides. Modified RNA containing pyrimidine-2′-fluoro-dNMPs, such as DuraScript® RNA made by in vitro transcription using the DuraScribe® T7 & SP6 Transcription Kits is completely resistant to cleavage by RNase A.

Applications

Removal of RNA from DNA preparations. Removal of unhybridized regions of RNA from DNA-RNA or RNA-RNA hybrids.

Concentration

5 mg/ml

Size/Form

10 mg

Storage

50% glycerol containing 25 mM sodium acetate (pH 4.6).

Publications

Q & A

Customer Reviews

Customer Q&As

Can this enzyme cleave all bases?

A: No, this enzyme is a ribonucleic acid-specific endonuclease, it specifically degrade single-stranded RNA at the C and U residues.

Can this enzyme be used to remove RNA from DNA samples?

A: Yes, it can be used to remove RNA from DNA preparations, and to remove the non-hybridized regions of RNA from DNA-RNA or RNA-RNA hybrids.

Are there any methods for preparing RNA samples that can avoid RNase A cleavage?

A: Yes, RNA produced through in vitro transcription using T7 and SP6 transcription reagent kits are completely resistant to RNase A cleavage.

Does the enzyme have activity in high salt systems?

A: Yes, RNase A has the highest activity for cleaving single-stranded RNA and is active under a variety of reaction conditions. At low salt concentrations (0-100 mM NaCl), it can be used to cleave single-stranded RNA, double-stranded RNA, and RNA chains formed by RNA-DNA hybrids. However, at high salt concentrations (≥0.3 M), RNase A specifically cleaves single-stranded RNA.

What is the enzyme concentration when using the enzyme?

A: The enzyme concentration varies depending on the sample and buffer system used. The enzyme is provided at a concentration of 5mg/ml, and the recommended working concentration is 1-100 μg/mL.

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Customer Reviews

Mismatched bases can be removed

Mismatched bases in RNA-RNA or RNA-DNA can be cleaved by RNaseA and can be used to determine the location of single base mutations in DNA or RNA.

United States

01/18/2023

Convenient to use

RNase A has the highest activity in cutting single-stranded RNA and is active in both low and high salt reaction systems.

United States

01/11/2023

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RNase A

Publications

Q & A

Customer Reviews

Can this enzyme cleave all bases?

Can this enzyme be used to remove RNA from DNA samples?

Are there any methods for preparing RNA samples that can avoid RNase A cleavage?

Does the enzyme have activity in high salt systems?

What is the enzyme concentration when using the enzyme?

Mismatched bases can be removed

Convenient to use

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