Transfected Stable Cell Lines
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Cat. No. : EMNT2027
| Cat. No. | EMNT2027 |
| Description | RNAse Inhibitor is an acidic, 52 kDa protein that is a potent non-competitive inhibitor of pancreatic-type ribonucleases such as RNase A, RNase B, and RNase C. The enzyme is provided as a fusion of the porcine RNAse Inhibitor gene with a proprietary, 22.5 kDa protein tag. |
| Source | A recombinant E. coli strain carrying the porcine RNAse Inhibitor gene. |
| Concentration | 40000 U/ml |
| Size | 20000 Units |
| Unit Definition | One unit is defined as the amount of enzyme required to inhibit by 50% the hydrolysis of cytidine 2",3"-cyclic monophosphate by 5 ng of RNAse A. |
| Storage | 20 mM Hepes-KOH 50 mM KCl 8 mM DTT 50% glycerol pH 7.5 at 25 °C |
A: This enzyme is a fusion protein that is expressed and purified by fusing the pig RNase inhibitor gene with a proprietary 22.5 kDa protein tag.
A: No, it can only bind and inhibit the activity of RNase A, RNase B, and RNase C through a non-competitive mechanism. RNase inhibitor cannot inhibit the enzymatic activity of RNase T1, T2, H, U1, U2, and CL3.
A: RNase inhibitor is an acidic protein that maintains its RNase inhibitory activity within the pH range of 5-8, and its enzymatic activity is highest at pH 7-8.
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We successfully used this product for the separation and purification of RNA in vitro translation and mRNA-protein complexes.
When the inhibitor is added to the reaction system, the final reaction concentration should reach 1U/ul.
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