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RNAse Inhibitor

For research use only. Not intended for any clinical use.
Cat.No.
EMNT2027
Description
RNAse Inhibitor is an acidic, 52 kDa protein that is a potent non-competitive inhibitor of pancreatic-type ribonucleases such as RNase A, RNase B, and RNase C. The enzyme is provided as a fusion of the porcine RNAse Inhibitor gene with a proprietary, 22.5 kDa protein tag.
Concentration
40000 U/ml
Size/Form
20000 Units
Source
A recombinant E. coli strain carrying the porcine RNAse Inhibitor gene.
Storage
20 mM Hepes-KOH 50 mM KCl 8 mM DTT 50% glycerol pH 7.5 at 25 °C
Unit Definition
One unit is defined as the amount of enzyme required to inhibit by 50% the hydrolysis of cytidine 2",3"-cyclic monophosphate by 5 ng of RNAse A.

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Q & A

Customer Reviews

Customer Q&As
Where does this enzyme come from?

A: This enzyme is a fusion protein that is expressed and purified by fusing the pig RNase inhibitor gene with a proprietary 22.5 kDa protein tag.

Can this product inhibit the activity of all RNases?

A: No, it can only bind and inhibit the activity of RNase A, RNase B, and RNase C through a non-competitive mechanism. RNase inhibitor cannot inhibit the enzymatic activity of RNase T1, T2, H, U1, U2, and CL3.

What is the optimal pH for the activity of this enzyme?

A: RNase inhibitor is an acidic protein that maintains its RNase inhibitory activity within the pH range of 5-8, and its enzymatic activity is highest at pH 7-8.

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Customer Reviews
Easy to use

We successfully used this product for the separation and purification of RNA in vitro translation and mRNA-protein complexes.

United States

06/16/2022

High activity

When the inhibitor is added to the reaction system, the final reaction concentration should reach 1U/ul.

United States

01/12/2023

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