Pvu Rts1I Endonuclease

Name: Pvu Rts1I Endonuclease
SKU: EMNT2018
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EMNT2018

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Product Information

Cat. No.	EMNT2018
Description	digestion conditions, the enzyme selectively cleaves DNA that contains the modified base on one or both strands. The consensus cleavage site of Pvu Rts1I is 5-hmCN11-12/N9-10G.2 Pvu Rts1I serves as a useful tool to analyze 5-hmC patterns within the genome. In addition to distinguishing between 5-mC and 5-hmC in ds DNA, the enzyme can also cleave both glucosylated and nonglucosylated 5-hmC ds DNA.The enzyme is available in a 100-unit size at a concentration of 1 U/μl. The enzyme is supplied with a 10X Reaction Buffer.
Concentration	1000 units/ml
Applications	Differentiation of 5-hmC from 5-mC. Cleavage of DNA at glucosylated and nonglucosylated 5-hmC.
Size	100 units
Unit Definition	One unit is the amount of Pvu Rts1I needed for complete digestion of 1 μg of T2 gt- DNA in 60 minutes at 30 °C in 1X Reaction Buffer, in a 50-μl reaction.
Storage	50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.

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Customer Q&As

Can Pvu Rts1I Endonuclease be used for clinical research?

A: Pvu Rts1I Endonuclease is for research use only and is not intended for any clinical applications.

Can Pvu Rts1I Endonuclease cleave glucose-modified dsDNA?

A: Pvu Rts1I Endonuclease can cleave both glucosylated and non-glucosylated 5-hmC dsDNA.

What is the concentration of Pvu Rts1I Endonuclease?

A: The concentration of Pvu Rts1I Endonuclease is 1000 units/ml.

Does Pvu Rts1I Endonuclease come with its own buffer?

A: Pvu Rts1I Endonuclease is supplied with a 10X reaction buffer.

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