digestion conditions, the enzyme selectively cleaves DNA that contains the modified base on one or both strands. The consensus cleavage site of Pvu Rts1I is 5-hmCN11-12/N9-10G.2 Pvu Rts1I serves as a useful tool to analyze 5-hmC patterns within the genome. In addition to distinguishing between 5-mC and 5-hmC in ds DNA, the enzyme can also cleave both glucosylated and nonglucosylated 5-hmC ds DNA.The enzyme is available in a 100-unit size at a concentration of 1 U/μl. The enzyme is supplied with a 10X Reaction Buffer.
Differentiation of 5-hmC from 5-mC. Cleavage of DNA at glucosylated and nonglucosylated 5-hmC.
One unit is the amount of Pvu Rts1I needed for complete digestion of 1 μg of T2 gt- DNA in 60 minutes at 30 °C in 1X Reaction Buffer, in a 50-μl reaction.
50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.