Exonuclease I (E.coli)

Name: Exonuclease I (E.coli)
SKU: EMQZ1418
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EMQZ1418

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Product Information

Cat. No.	EMQZ1418
Description	Exonuclease I (ExoI) degrades single-stranded DNA in a 3' to 5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact. It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups.
Source	E. coli strain
Concentration	20,000 U/ml
Applications	• Primer removal from PCR mixtures • Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures • Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus
Size	3,000 U; 15,000 U
Storage	Store at -20°C

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Customer Reviews

Customer Q&As

What experiments is this product suitable for?

A: This product can be used in experiments such as removing primers from PCR mixtures, removing single-stranded DNA with 3'-hydroxyl ends from nucleic acid mixtures, and detecting the presence of single-stranded DNA with 3'-hydroxyl ends.

Can this enzyme be used for double-stranded DNA?

A: No, this enzyme cannot digest double-stranded DNA, so single-stranded DNA with secondary structures needs to be denatured before complete digestion can occur.

Do I need to contact the staff after purchasing?

A: Generally, no. Orders will be automatically placed and delivered by the platform. However, if there are any other requests or questions, customers can contact the staff for inquiries.

Does the product come with a buffer solution included?

A: Yes, there is. After receiving the product, there will be one tube of enzyme and one tube of 10× buffer. When using, dilute the buffer to 1×.

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Customer Reviews

High product quality

It does not cut DNA chains with blocked 3'-OH groups by phosphate or acetyl groups.

United States

2022-03-25

Product quality is good.

There have been no problems with the product during its shelf life, and the success rate of experiments is high.

United States

2022-06-16

Simple program

It is generally reacted at 37°C and then inactivated at 80°C for 20 minutes.

United States

2023-01-12

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Exonuclease I (E.coli)

Product Information

Q & A

Customer Reviews

What experiments is this product suitable for?

Can this enzyme be used for double-stranded DNA?

Do I need to contact the staff after purchasing?

Does the product come with a buffer solution included?

High product quality

Product quality is good.

Simple program

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