Exonuclease VII has high enzymatic specificity for ssDNA and exhibits both 5′→3′ and 3′→5′ exonuclease activities. It is useful for rapid removal of single-stranded oligonucleotide primers from a completed PCR when different primers are required for subsequent PCR amplifications. Exonuclease VII digestion of ssDNA occurs in the absence of magnesium. Exonuclease VII can be inactivated by heating at 95 °C for 10 minutes.
Removal of single-stranded oligonucleotide primers after PCR. Minimize the effect of primers left over from previous PCR amplifications.
One unit of Exonuclease VII catalyzes the release of 1 nmol of acid-soluble nucleotides from activated heat denatured calf thymus DNA in 30 minutes at 37 °C under standard assay conditions.
500 units; 100 units
50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 0.1 M NaCl, and 0.1% Triton® X-100.