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Exonuclease VII

Cat.No.
EMNT2010
Description
Exonuclease VII has high enzymatic specificity for ssDNA and exhibits both 5′→3′ and 3′→5′ exonuclease activities. It is useful for rapid removal of single-stranded oligonucleotide primers from a completed PCR when different primers are required for subsequent PCR amplifications. Exonuclease VII digestion of ssDNA occurs in the absence of magnesium. Exonuclease VII can be inactivated by heating at 95 °C for 10 minutes.
Applications
Removal of single-stranded oligonucleotide primers after PCR. Minimize the effect of primers left over from previous PCR amplifications.
Unit Definition
One unit of Exonuclease VII catalyzes the release of 1 nmol of acid-soluble nucleotides from activated heat denatured calf thymus DNA in 30 minutes at 37 °C under standard assay conditions.
Concentration
10000 units/ml
Size
500 units; 100 units
Storage Buffer
50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 0.1 M NaCl, and 0.1% Triton® X-100.
Related Products
Cat#
Product Name
Recognition Site
Size
Price
EMNT2027
N/A
20000 Units


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USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-biogene.com

Europe
Tel: 44-207-097-1828
Email: info@creative-biogene.com
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CONTACT CREATIVE BIOGENE

45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-biogene.com

Europe
Tel: 44-207-097-1828

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