Exonuclease VII

Name: Exonuclease VII
SKU: EMNT2010
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EMNT2010

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Product Information

Cat. No.	EMNT2010
Description	Exonuclease VII has high enzymatic specificity for ssDNA and exhibits both 5′→3′ and 3′→5′ exonuclease activities. It is useful for rapid removal of single-stranded oligonucleotide primers from a completed PCR when different primers are required for subsequent PCR amplifications. Exonuclease VII digestion of ssDNA occurs in the absence of magnesium. Exonuclease VII can be inactivated by heating at 95 °C for 10 minutes.
Concentration	10000 units/ml
Applications	Removal of single-stranded oligonucleotide primers after PCR. Minimize the effect of primers left over from previous PCR amplifications.
Size	500 units; 100 units
Unit Definition	One unit of Exonuclease VII catalyzes the release of 1 nmol of acid-soluble nucleotides from activated heat denatured calf thymus DNA in 30 minutes at 37 °C under standard assay conditions.
Storage	50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 0.1 M NaCl, and 0.1% Triton® X-100.

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Customer Q&As

What is the shelf life of the Exonuclease VII?

A: The Exonuclease VII should be stored at -20°C and is stable for 2 years.

Does the Exonuclease VII have activity against double-stranded DNA?

A: No, Exonuclease VII does not have activity on linear or circular double-stranded DNA under the conditions tested.

What is the specification of the Exonuclease VII?

A: The Exonuclease VII is available in two sizes: 250U and 2500U, and you can choose the one that suits your experimental needs.

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