Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RI0199
Host Cell : CHO Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RI0199 |
| Description | This cell line is engineered to overexpress human TRPM8. |
| Background | The human TRPM8 gene encodes the pore-forming subunit of TRPM8, a non-selective, cation-permeable channel. TRPM8 channels are therapeutic targets in cancer, overactive bladder, and neuropathic pain. |
| Target Gene | TRPM8 |
| Gene Species | Homo sapiens (Human) |
| Abbr | CHO-HuTRPM8 |
| Alias | TRPM8, trp-p8, LTrpC-6, TRPP8, LTRPC6, MGC2849 |
| Host Cell | CHO |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Applications |
1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | TRPM8 transient receptor potential cation channel, subfamily M, member 8 [ Homo sapiens ] |
| Gene Symbol | TRPM8 |
| Synonyms | TRPP8; LTRPC6 |
| Gene Description | transient receptor potential cation channel, subfamily M, member 8 |
| GeneID | 79054 |
| Uni ProtID | Q3YFM7 |
| mRNA Refseq | NM_024080.4 |
| Protein Refseq | NP_076985.4 |
| Chromosome Location | 2q37.1 |
| Function | calcium channel activity; protein homodimerization activity; |
| MIM | 606678 |
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
We use the TRPM8 CHO cells for screening novel modulators, and their sensitivity is outstanding. They respond beautifully to menthol and thermal stimuli with great reproducibility. The cell morphology and growth rates are perfectly normal, making them incredibly easy to maintain.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.