Human TRPM8 Stable Cell Line - HEK293

Name: Human TRPM8 Stable Cell Line - HEK293
SKU: CSC-RI00215

Cat.No. : CSC-RI00215 Host Cell: HEK293

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Cell Line Information

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Cat. No.	CSC-RI00215
Description	This is a stable cell line that is engineered to constitutively express human transient receptor potential cation channel subfamily M member 8 (TRPM8) in HEK293 cells. The expression of human TRPM8 in this cell line has been validated by real-time qPCR. This cell line is a valuable cell model for investigating human TRPM8 functions and screening assays.
Gene	TRPM8
Gene Species	Human
Host Cell	HEK293
Host Cell Species	Human embryonic kidney cell line
Stability	This cell line is stable at least 10 passages.
Product Type	Human gene overexpression stable cell line
Applications	1) studying the functional characteristics of TRPM8, such as its activation by cold temperatures and specific agonists (e.g., menthol), as well as its ion conductance properties. 2) investigating the role of TRPM8 in pain signaling, particularly cold-induced pain, by allowing researchers to observe downstream signaling pathways upon TRPM8 activation. 3) identification and evaluation of potential TRPM8 agonists, antagonists, or modulators that may have therapeutic implications for conditions like chronic pain, overactive bladder, or prostate cancer. 4) studying the interactions between TRPM8 and other proteins or cellular components, contributing to a better understanding of its regulatory mechanisms in various physiological and pathological processes.
Quality Control	1) detection of gene mRNA overexpression level by real-time qPCR 2) mycoplasma detection
Size Form	One vial of frozen cells, typically >1x10^6cells/vial
Shipping	Dry ice
Storage	Liquid nitrogen

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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