Human TRPC6 Stable Cell Line - CHO

TRPC6 (Transient Receptor Potential Canonical 6) is a receptor-activated, nonselective cation channel belonging to the classical TRPC subfamily. Encoded by the TRPC6 gene on human chromosome 11, it forms a homotetrameric channel that responds directly to diacylglycerol (DAG), a product of phospholipase C-mediated signaling. Upon activation, TRPC6 mediates calcium and sodium influx across the plasma membrane, leading to cellular depolarization and the activation of downstream calcium-dependent pathways. The channel is widely expressed in vascular smooth muscle, podocytes in the kidney, and various regions of the central nervous system, where it plays critical roles in regulating vascular tone, glomerular filtration barrier function, and synaptic plasticity. The Human TRPC6 Stable Cell Line - CHO is generated by stably transfecting TRPC6 into Chinese hamster ovary (CHO) cells, creating a reliable and reproducible cell-based tool that stably expresses functional human TRPC6 channels at the plasma membrane. This engineered cell line provides a consistent and homogeneous expression system, making it well suited for studying channel pharmacology, electrophysiology, and signal transduction in a controlled in vitro setting.

This stable cell line serves as a valuable platform for a range of research applications, particularly in ion channel drug discovery and target validation. It can be used in high-throughput screening campaigns to identify novel TRPC6 agonists, antagonists, or modulators, using fluorescence-based membrane potential or calcium influx assays. The line is also suitable for patch-clamp electrophysiology studies to characterize channel kinetics, ion selectivity, and voltage-dependent properties. Given the established involvement of TRPC6 in diseases such as focal segmental glomerulosclerosis (FSGS), pulmonary hypertension, cardiac hypertrophy, and certain neurological disorders, this cell line enables researchers to evaluate the functional impact of disease-associated mutations, test the selectivity of candidate compounds, and investigate the molecular mechanisms underlying channel regulation. Additionally, it can be used for downstream signaling studies, including the assessment of DAG-dependent activation, store-operated calcium entry, and interactions with binding partners such as podocin or nephrin, making it a versatile tool for both basic and translational research.