Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RI00216
Host Cell : CHO Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RI00216 |
| Description | This is a stable cell line that is engineered to constitutively express human transient receptor potential cation channel subfamily C member 6 (TRPC6) in HEK293 cells. The expression of human TRPC6 in this cell line has been validated by real-time qPCR. This cell line is a valuable cell model for investigating human TRPC6 functions and screening assays. |
| Product Type | Human gene overexpression stable cell line |
| Target Gene | TRPC6 |
| Gene Species | Human |
| Host Cell | CHO |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Applications |
1) studying the functional properties of TRPC6, including its ion channel activity, activation mechanisms. 2) investigating the role of TRPC6 in various physiological and pathological processes, such as vascular smooth muscle contraction, podocyte function in the kidney, and cardiac hypertrophy. 3) drug discovery and screening assays to identify and evaluate potential TRPC6 modulators (agonists, antagonists, or regulators), which may have therapeutic potential for diseases associated with TRPC6 dysregulation, such as focal segmental glomerulosclerosis (FSGS), hypertension, or cardiac diseases. 4) studying the interactions between TRPC6 and other proteins (e.g., signaling molecules, cytoskeletal components) to elucidate the molecular pathways that TRPC6 participates in or regulates. |
| Size | One vial of frozen cells, typically >1x10^6cells/vial |
| Stability | This cell line is stable at least 10 passages. |
| Quality Control |
1) detection of gene mRNA overexpression level by real-time qPCR 2) mycoplasma detection |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Target Gene | TRPC6 |
| Background | The protein encoded by this gene forms a receptor-activated calcium channel in the cell membrane. The channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation of this channel occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium. Defects in this gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2). [provided by RefSeq, Mar 2009] |
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We purchased the Human TRPC6 CHO cell line for our intracellular calcium assay screening. The cells showed fantastic stability and a highly consistent response to agonists across multiple passages. Creative Biogene has saved our lab immense time in assay development.
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