Pages
Products

Human TRPC6 Stable Cell Line - CHO

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RI00216

Host Cell :   CHO Size :   >1x106 frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RI00216
Description This is a stable cell line that is engineered to constitutively express human transient receptor potential cation channel subfamily C member 6 (TRPC6) in HEK293 cells. The expression of human TRPC6 in this cell line has been validated by real-time qPCR. This cell line is a valuable cell model for investigating human TRPC6 functions and screening assays.
Product Type Human gene overexpression stable cell line
Target Gene TRPC6
Gene Species Human
Host Cell CHO
Host Cell Species Cricetulus griseus (Chinese hamster)
Applications 1) studying the functional properties of TRPC6, including its ion channel activity, activation mechanisms.
2) investigating the role of TRPC6 in various physiological and pathological processes, such as vascular smooth muscle contraction, podocyte function in the kidney, and cardiac hypertrophy.
3) drug discovery and screening assays to identify and evaluate potential TRPC6 modulators (agonists, antagonists, or regulators), which may have therapeutic potential for diseases associated with TRPC6 dysregulation, such as focal segmental glomerulosclerosis (FSGS), hypertension, or cardiac diseases.
4) studying the interactions between TRPC6 and other proteins (e.g., signaling molecules, cytoskeletal components) to elucidate the molecular pathways that TRPC6 participates in or regulates.
Size One vial of frozen cells, typically >1x10^6cells/vial
Stability This cell line is stable at least 10 passages.
Quality Control 1) detection of gene mRNA overexpression level by real-time qPCR
2) mycoplasma detection
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Target Gene TRPC6
Background The protein encoded by this gene forms a receptor-activated calcium channel in the cell membrane. The channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation of this channel occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium. Defects in this gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2). [provided by RefSeq, Mar 2009]
Quick Inquiry

Q & A

Customer Reviews

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Customer Reviews
Save Time

We purchased the Human TRPC6 CHO cell line for our intracellular calcium assay screening. The cells showed fantastic stability and a highly consistent response to agonists across multiple passages. Creative Biogene has saved our lab immense time in assay development.

Germany

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Needs improvement

Satisfaction

General satisfaction

Very satisfaction