Cat.No. : CSC-RI0148 Host Cell: CHO-K1
| Cat. No. | CSC-RI0148 |
| Description | This cell line is engineered to overexpress human KCNQ4. |
| Background | The human KCNQ4 gene encodes the pore-forming subunit of Kv7.4, a voltage-gated potassium channel. KCNQ4 loss-of-function mutations cause hereditary deafness. Kv7.4 channels expressed in neurons are therapeutic targets in seizure. |
| Gene | KCNQ4 |
| Gene Species | Homo sapiens (Human) |
| Alias | KCNQ4, DFNA2, Kv7.4, KV7.4, DFNA2A |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Morphology | Epithelial-like |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Optimization of the expression vector, such as changing the promoter or enhancer sequences, or adjusting the culture conditions like temperature, CO2 concentration, and medium composition, can be attempted to increase protein expression.
A: Stable expressing cell clones can be selected and maintained using limiting dilution cloning or resistance markers and selection pressure, such as G418.
A: Operate in a sterile environment, regularly check for microbial contamination, use antibiotics to prevent contamination, and regularly sterilize culture equipment.
A: Potential reasons include cell aging due to over-passaging, insufficient nutrient in the culture medium, cell contamination, or inappropriate culture conditions.
A: Optimize the freezing and thawing procedures, use efficient cryopreservation media, slow cooling and rapid thawing, and transfer cells to pre-warmed culture medium immediately after thawing.
A: To ensure genetic stability in Human KCNQ4 Stable Cell Line-CHO after genetic manipulation, employ CRISPR/Cas9 for site-specific integration into genomic "safe harbors" that are resistant to gene silencing and maintain high transcriptional activity
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