Human KCNQ4 Stable Cell Line-CHO-K1

Name: Human KCNQ4 Stable Cell Line-CHO-K1
SKU: CSC-RI0148
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0148

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0148
Description	This cell line is engineered to overexpress human KCNQ4.
Background	The human KCNQ4 gene encodes the pore-forming subunit of Kv7.4, a voltage-gated potassium channel. KCNQ4 loss-of-function mutations cause hereditary deafness. Kv7.4 channels expressed in neurons are therapeutic targets in seizure.
Target Gene	KCNQ4
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuKCNQ4
Alias	KCNQ4, DFNA2, Kv7.4, KV7.4, DFNA2A
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent
Morphology	Epithelial-like

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	KCNQ4 potassium voltage-gated channel, KQT-like subfamily, member 4 [ Homo sapiens ]
Gene Symbol	KCNQ4
Synonyms	DFNA2; KV7.4; DFNA2A
Gene ID	9132
Uni Prot ID	B3KQH8
m RNA Refseq	NM_004700.3
Protein Refseq	NP_004691.2
Chromosome Location	1p34
Function	potassium channel activity; voltage-gated potassium channel activity;
Pathway	Cholinergic synapse, organism-specific biosystem; Neuronal System, organism-specific biosystem; Potassium Channels, organism-specific biosystem; Voltage gated Potassium channels, organism-specific biosystem;
MIM	603537

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Q & A

Customer Reviews

Customer Q&As

What should be done if insufficient protein expression is observed in the Human KCNQ4 Stable Cell Line-CHO?

A: Optimization of the expression vector, such as changing the promoter or enhancer sequences, or adjusting the culture conditions like temperature, CO2 concentration, and medium composition, can be attempted to increase protein expression.

How can the stability of the Human KCNQ4 Stable Cell Line-CHO be improved to maintain consistent protein expression during long-term culture?

A: Stable expressing cell clones can be selected and maintained using limiting dilution cloning or resistance markers and selection pressure, such as G418.

How to control contamination risks during large-scale production using the Human KCNQ4 Stable Cell Line-CHO?

A: Operate in a sterile environment, regularly check for microbial contamination, use antibiotics to prevent contamination, and regularly sterilize culture equipment.

What could be the reasons for a sudden decrease in the growth rate of the Human KCNQ4 Stable Cell Line-CHO?

A: Potential reasons include cell aging due to over-passaging, insufficient nutrient in the culture medium, cell contamination, or inappropriate culture conditions.

How to improve the viability of Human KCNQ4 Stable Cell Line-CHOafter thawing from cryopreservation?

A: Optimize the freezing and thawing procedures, use efficient cryopreservation media, slow cooling and rapid thawing, and transfer cells to pre-warmed culture medium immediately after thawing.

How to ensure genetic stability in the Human KCNQ4 Stable Cell Line-CHO after genetic manipulation?

A: To ensure genetic stability in Human KCNQ4 Stable Cell Line-CHO after genetic manipulation, employ CRISPR/Cas9 for site-specific integration into genomic "safe harbors" that are resistant to gene silencing and maintain high transcriptional activity

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