Human KCNMA1/KCNMB1 Stable Cell Line-CHO-K1

Name: Human KCNMA1/KCNMB1 Stable Cell Line-CHO-K1
SKU: CSC-RI0106
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0106

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0106
Description	This cell line is engineered to overexpress human KCNMB1.
Background	The KCNM1 gene encodes the pore-forming subunit of a large-conductance (260 pS single channel conductance), K+ -selective, channel that is activated by membrane depolarization and increased intracellular [Ca2+].
Target Gene	KCNMB1
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuKCNMA1/KCNMB1
Alias	KCNMB1, BKbeta, hbeta1, BKbeta1, K(VCA)beta
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent
Morphology	Epithelial-like

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	KCNMB1 potassium large conductance calcium-activated channel, subfamily M, beta member 1 [ Homo sapiens ]
Gene Symbol	KCNMB1
Synonyms	SLO-BETA; hslo-beta; K(VCA)beta
Gene Description	potassium large conductance calcium-activated channel, subfamily M, beta member 1
Gene ID	3779
Uni Prot ID	Q16558
m RNA Refseq	NM_004137.2
Protein Refseq	NP_004128.1
Chromosome Location	5q34
Function	calcium-activated potassium channel activity; potassium channel regulator activity;
Pathway	Ca2+ activated K+ channels, organism-specific biosystem; Hemostasis, organism-specific biosystem; Neuronal System, organism-specific biosystem; Nitric oxide stimulates guanylate cyclase, organism-specific biosystem; Platelet homeostasis, organism-specific biosystem; Potassium Channels, organism-specific biosystem; Vascular smooth muscle contraction, organism-specific biosystem;
MIM	603951

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Q & A

Customer Reviews

Customer Q&As

How was the Human KCNMA1/KCNMB1 Stable Cell Line-CHO established?

A: The cell line was established by stably transfecting the human KCNMA1 (large conductance calcium-activated potassium channel alpha subunit) and KCNMB1 (beta subunit) genes into CHO cells.

What are the main applications of this cell line in cardiology research?

A: It is primarily used to study the role of KCNMA1/KCNMB1 in cardiac cell electrophysiology and rhythm regulation.

What are the applications of the Human KCNMA1/KCNMB1 Stable Cell Line-CHO in neuroscience research?

A: It can be used to study the role of these channels in regulating neuronal excitability and neural signal transduction.

What should be noted when using the Human KCNMA1/KCNMB1 Stable Cell Line-CHO for drug screening?

A: Special attention should be paid to the impact of drugs on KCNMA1/KCNMB1 channel activity and the resulting changes in cell electrophysiological properties.

What are the potential uses of the Human KCNMA1/KCNMB1 Stable Cell Line-CHO in model studies?

A: It can be used to establish cellular models of cardiac and neurological diseases, especially those related to dysfunctions of the KCNMA1/KCNMB1 channels.

What special considerations are there for maintaining the Human KCNMA1/KCNMB1 Stable Cell Line-CHO?

A: Regular monitoring of cell division and gene expression is necessary to ensure stable expression of the channel genes and consistency in cell characteristics.

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