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Cat.No. : CSC-RI0029 Host Cell: CHO-K1
| Cat. No. | CSC-RI0029 |
| Description | This cell line is engineered to overexpress human KCNA4. |
| Background | Kv1.4 is a voltage-gated, fast-inactivating, A-type potassium ion channel also known as HuK(II), hPCN2, HK1, RCK4, RHK1, RK4, RK8 and MK4. It is expressed in the CNS, lung, skeletal muscle, heart and pancreatic islet cells. It is responsibly for neuronal after hypolarization. The expression of Kv1.4 has been shown to increase after induction of diabetes and in ventricular myocytes after a heart attack. |
| Gene | KCNA4 |
| Gene Species | Homo sapiens (Human) |
| Alias | KCNA4, KCNA4L, HK1, HPCN2, Kv1.4, HBK4, PCN2, HUKII, KCNA8, KV1.4, KCNA4L |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Morphology | Epithelial-like |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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