Human KCNQ4 Stable Cell Line-HEK293

Name: Human KCNQ4 Stable Cell Line-HEK293
SKU: CSC-RI0045
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0045

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0045
Description	This cell line is engineered to overexpress human KCNQ4.
Target Gene	KCNQ4
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuKCNQ4
Alias	KCNQ4, DFNA2, Kv7.4, KV7.4, DFNA2A
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	KCNQ4 potassium voltage-gated channel, KQT-like subfamily, member 4 [ Homo sapiens ]
Gene Symbol	KCNQ4
Synonyms	DFNA2; KV7.4; DFNA2A
Gene ID	9132
Uni Prot ID	B3KQH8
m RNA Refseq	NM_004700.3
Protein Refseq	NP_004691.2
Chromosome Location	1p34
Function	potassium channel activity; voltage-gated potassium channel activity;
Pathway	Cholinergic synapse, organism-specific biosystem; Neuronal System, organism-specific biosystem; Potassium Channels, organism-specific biosystem; Voltage gated Potassium channels, organism-specific biosystem;
MIM	603537

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KCNQ genes encode potassium channels that have been associated with both cardiac and hearing abnormalities in humans, most notably the KNCQ1 gene. Using a partial KCNQ3 cDNA Kubisch et al. screened a human retinal cDNA lambda phage library and obtained a novel homolog they named KCNQ4. When expressed in Xenopus oocytes KCNQ4 encodes a potassium current inhibited by 30% in the presence of 200 μM linopridine, whereas the current due to KCNQ3/KCNQ4 heteromers in the same study was inhibited by 75% at that concentration. Kubisch et al. also mapped the KCNQ4 gene to the 1p34 human chromosome, in a region associated with autosomal dominant deafness DFNA2A. Subsequent studies have identified additional families with mutations in KCNQ4 and hearing loss. KCNQ4-specific openers could possibly treat progressive hearing loss and tinnitus Kubisch et al..

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