Human KCNJ11/MED23 Stable Cell Line-HEK293

Name: Human KCNJ11/MED23 Stable Cell Line-HEK293
SKU: CSC-RI0025
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0025

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Cat. No.	CSC-RI0025
Description	This cell line is engineered to overexpress human KCNJ11/MED23.
Background	Molecular cloning has identified the channel as a heteromer comprised of four pore-forming subunits, termed Kir 6.2, and four auxiliary sulphonylurea receptor subunits, Sur2A. The channel is gated by intracellular ATP and NDP while the Sur2A is regulated by both agonists, such as the potassium channel opener pinacidil, and antagonists, such as glibenclamide. In vivo the cardiac KATP channel is thought to be cardioprotective during periods of ischemic stress, as activation of the channel hyperpolarizes the cell, reducing calcium channel activity, leading to a shortening of the action potential duration.
Target Gene	KCNJ11/MED23
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuKCNJ11/MED23
Alias	MED23, SUR2, CRSP3, MRT18, SUR-2, ARC130
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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